Overexpressed microRNA-136 works as a cancer suppressor in gallbladder cancer through suppression of JNK signaling pathway via inhibition of MAP2K4

American Journal of Physiology. Gastrointestinal and Liver Physiology
Jixiang NiuFuzhou Li

Abstract

In recent studies, microRNAs (miRs) have been widely explored as important regulator in tumor suppression. MiR-136 has been suggested to participate in tumor inhibition through control of vital cellular processes, such as angiogenesis, proliferation and apoptosis. This study aimed to evaluate the effects of overexpressed miR-136 by transferring mimics in gallbladder cancer (GBC) and to assess the functional role of miR-136 in GBC cell behaviors with the involvement of the MAP2K4-dependent JNK signaling pathway. Differentially expressed miRs associated with GBC were screened using microarray expression profiles, which identified that miR-136 expression was decreased in GBC. Furthermore, MAP2K4 was validated as a target gene of miR-136. In order to uncover functional relevance regarding miR-136 and MAP2K4 in GBC, cultured GBC cell lines were prepared to transfect with mimic, inhibitor, siRNA or vectors. At the same time, the transfected GBC cells were inoculated into nude mice to validate findings in vivo. The obtained results demonstrated that overexpressed miR-136 inhibited angiogenesis, cell proliferation and promoted apoptosis in GBC cell lines in vitro, accompanied by impeded cellular tumorigenicity in nude mice via the supp...Continue Reading

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Citations

Mar 25, 2020·World Journal of Gastroenterology : WJG·Hong ZhengYong-Lin Yu
Mar 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Jun 17, 2021·Molecular Medicine Reports·Qi Zheng, Wei Hou

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Datasets Mentioned

BETA
GSE104165

Methods Mentioned

BETA
PCR
transfection
xenograft
confocal microscopy
transfect

Software Mentioned

pheatmap
cytoscape
R language limma
ImageJ
SPSS

Related Concepts

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Apoptosis

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