Overexpressing CYP71Z2 enhances resistance to bacterial blight by suppressing auxin biosynthesis in rice

PloS One
Wenqi LiWeigong Zhong

Abstract

The hormone auxin plays an important role not only in the growth and development of rice, but also in its defense responses. We've previously shown that the P450 gene CYP71Z2 enhances disease resistance to pathogens through regulation of phytoalexin biosynthesis in rice, though it remains unclear if auxin is involved in this process or not. The expression of CYP71Z2 was induced by Xanthomonas oryzae pv. oryzae (Xoo) inoculation was analyzed by qRT-PCR, with GUS histochemical staining showing that CYP71Z2 expression was limited to roots, blades and nodes. Overexpression of CYP71Z2 in rice durably and stably increased resistance to Xoo, though no significant difference in disease resistance was detected between CYP71Z2-RNA interference (RNAi) rice and wild-type. Moreover, IAA concentration was determined using the HPLC/electrospray ionization/tandem mass spectrometry system. The accumulation of IAA was significantly reduced in CYP71Z2-overexpressing rice regardless of whether plants were inoculated or not, whereas it was unaffected in CYP71Z2-RNAi rice. Furthermore, the expression of genes related to IAA, expansin and SA/JA signaling pathways was suppressed in CYP71Z2-overexpressing rice with or without inoculation. These results...Continue Reading

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Citations

Feb 19, 2016·Plant Cell Reports·Prince MarowaYingzhen Kong
Sep 25, 2017·The Plant Journal : for Cell and Molecular Biology·Sang-Ho ParkAiming Wang
Oct 4, 2021·Functional Plant Biology : FPB·Zhe-Xin LiYi-Qing Liu

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Methods Mentioned

BETA
transgenic
PCR
electron microscopy

Software Mentioned

MEGA
MEGA ( Molecular evolutional genetics analysis

Related Concepts