Overexpressing the H-protein of the glycine cleavage system increases biomass yield in glasshouse and field-grown transgenic tobacco plants

Plant Biotechnology Journal
Patricia E Lopez-CalcagnoChristine A Raines

Abstract

Photorespiration is essential for C3 plants, enabling oxygenic photosynthesis through the scavenging of 2-phosphoglycolate. Previous studies have demonstrated that overexpression of the L- and H-proteins of the photorespiratory glycine cleavage system results in an increase in photosynthesis and growth in Arabidopsis thaliana. Here, we present evidence that under controlled environment conditions an increase in biomass is evident in tobacco plants overexpressing the H-protein. Importantly, the work in this paper provides a clear demonstration of the potential of this manipulation in tobacco grown in field conditions, in two separate seasons. We also demonstrate the importance of targeted overexpression of the H-protein using the leaf-specific promoter ST-LS1. Although increases in the H-protein driven by this promoter have a positive impact on biomass, higher levels of overexpression of this protein driven by the constitutive CaMV 35S promoter result in a reduction in the growth of the plants. Furthermore in these constitutive overexpressor plants, carbon allocation between soluble carbohydrates and starch is altered, as is the protein lipoylation of the enzymes pyruvate dehydrogenase and alpha-ketoglutarate complexes. Our data...Continue Reading

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Citations

Feb 5, 2019·Journal of Experimental Botany·Armin Kubis, Arren Bar-Even
May 3, 2019·Plant, Cell & Environment·Rebecca A Slattery, Donald R Ort
Nov 7, 2019·Nature·Julia Bailey-SerresJulian I Schroeder
Oct 19, 2019·The Plant Journal : for Cell and Molecular Biology·Michele Faralli, Tracy Lawson
Dec 6, 2019·The Plant Journal : for Cell and Molecular Biology·Andrew J SimkinTracy Lawson
Jan 7, 2020·The Plant Journal : for Cell and Molecular Biology·Alisdair R Fernie, Hermann Bauwe
Apr 11, 2020·Journal of Experimental Botany·Stefan Timm, Martin Hagemann
Jul 2, 2020·Plants·Lisa RotaspertiPaolo Pesaresi
Aug 1, 2020·Frontiers in Plant Science·Marouane BaslamÁlvaro Sanz-Sáez
Aug 13, 2020·Nature Plants·Patricia E López-CalcagnoChristine A Raines
Dec 29, 2020·Plant Communications·Willian Batista-SilvaWagner L Araújo
Dec 11, 2020·Journal of Experimental Botany·Jonathan Przybyla-ToscanoNicolas Rouhier
Feb 24, 2021·Journal of Experimental Botany·Caitlin E MooreAmanda P Cavanagh
Oct 16, 2020·Scientific Reports·José G VallarinoAlisdair R Fernie
Mar 3, 2021·Journal of Experimental Botany·Paula da Fonseca-PereiraWagner L Araújo
Mar 1, 2021·Journal of Experimental Botany·José L ArausRubén Vicente
Jun 15, 2021·Frontiers in Microbiology·Sanjana KaulManoj K Dhar
Oct 9, 2021·Biochemical Society Transactions·Concepción IñiguezJeroni Galmés

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Methods Mentioned

BETA
transgenic
lipoylation
transgenics
reduced
PCR

Software Mentioned

ImageJ
SPSS ( Statistical Product and Service Solutions )
R

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