Overexpression in colorectal carcinoma of two lysosomal enzymes, CLN2 and CLN1, involved in neuronal ceroid lipofuscinosis

Cancer
Toshihiko TsukamotoFumio Konishi

Abstract

Lysosomal proteases are implicated in cancer progression and metastasis. In the current study, using subtraction cloning for genes that are differentially expressed in metastasis, the authors isolated a clone encoding ceroid lipofuscinosis, neuronal 2 (CLN2), which is a lysosomal serine protease defective in neuronal ceroid lipofuscinosis (NCL). Increased CLN2 activity has been reported in breast carcinoma and the antiapoptotic effect of another causative gene of NCL, ceroid lipofuscinosis, neuronal 1 (CLN1), is known. The mRNA levels of CLN2, CLN1, and cathepsins B, D, H, and L were investigated in colorectal carcinoma patients with different clinical stages using real-time quantitative reverse transcriptase polymerase chain reaction. A polyclonal antibody was raised against a recombinant CLN2 protein for immunoblotting and immunohistochemistry. The mRNA levels of CLN1 and cathepsins B, D, and L were significantly higher in metastatic lesions than in primary tumors. In the primary tumors, mRNA expressions of CLN2 and cathepsin D were associated with advanced clinical stages (P < .015 and P < .031, respectively). Among the lysosomal enzymes examined, only the mRNA expression of CLN2 in both the primary tumors of all patients an...Continue Reading

References

Sep 30, 1991·International Journal of Cancer. Journal International Du Cancer·S ShujaM J Murnane
Jun 22, 1999·Human Pathology·K HiraiS Tanaka
Feb 7, 2001·British Journal of Cancer·E C del ReM J Murnane
Sep 11, 2001·Neurology·K E WisniewskiM Philippart
Nov 1, 2002·The International Journal of Biological Markers·Y KanberN Aydin
Feb 3, 2004·Current Pharmaceutical Design·Michael J Duffy
Mar 5, 2004·Brain Pathology·Sara E Mole
Mar 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Izabela Berdowska
Jun 16, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A M TroyD P O'Donoghue

❮ Previous
Next ❯

Citations

Oct 13, 2006·The Journal of Biological Chemistry·Bassam HaidarYves L Marcel
Jul 20, 2017·NPJ Breast Cancer·Alison M Anderson, Mark A Ragan
Apr 24, 2021·IScience·Shyong Quan YapRobert Joseph Huber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Batten Disease

Batten Disease is a group of nervous system disorders known as neuronal ceroid lipofuscinosis. This feed focuses on neurobiological and neuropathological aspects of this disease.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.