Abstract
Microbial oil triacylglycerol (TAG) from the renewable feedstock attract much attention. The oleaginous yeast Yarrowia lipolytica has become the most studied for lipid biosynthesis. Fatty acid desaturases catalyze the introduction of a double bond into fatty-acid hydrocarbon chains to produce unsaturated fatty acids. Desaturases are known to enhance lipid accumulation. In this study, we have achieved a significant increase in lipid production and increase the unsaturated fatty acids content in Y. lipolytica. By comparing the expression of the native genes of △-9 stearoyl-CoA desaturase (SCD) and △12 desaturase (△12D), and an exogenous △15 desaturase (△15D) from flax in the strain with deleted peroxisomal biogenesis factor 10 (PEX10) and overexpressed diacylglyceride acyl-transferase (DGA1), we found that the strain with overexpressed △15 desaturase accumulated 30.7% lipid. Simultaneously, we explored the effect of two copies of desaturase genes (12D-SCD, 15D-SCD, 12D-15D) on lipid production, and found co-expression of △12D and △15D accumulated 42.6% lipid. The lipid content was further increased by 56.3% through the deletion of the multifunctional enzyme (MFE1) and the overexpression of acetyl-CoA carboxylase (ACC1). Finally, ...Continue Reading
References
Apr 24, 1999·European Journal of Biochemistry·E SakuradaniS Shimizu
Nov 11, 2003·Journal of Microbiological Methods·P FickersJ M Nicaud
Jun 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Howard G DamudeNarendra S Yadav
Feb 10, 2007·Science·Gregory Stephanopoulos
Oct 28, 2008·Applied and Environmental Microbiology·Athanasios BeopoulosJean-Marc Nicaud
Mar 2, 2010·New Biotechnology·Lu-Te ChuangYung-Sheng Huang
May 24, 2011·Applied Microbiology and Biotechnology·Ramdane HaddoucheJean-Marc Nicaud
May 31, 2011·Metabolic Engineering·Thierry Dulermo, Jean-Marc Nicaud
Nov 2, 2011·The Journal of Biological Chemistry·Xue-Rong ZhouSurinder P Singh
Oct 3, 2012·Metabolic Engineering·Mitchell Tai, Gregory Stephanopoulos
Apr 23, 2013·Journal of Biotechnology·John BlazeckHal S Alper
Jul 31, 2013·Biotechnology Letters·Huaiyuan ZhangWei Chen
Oct 19, 2013·Applied Microbiology and Biotechnology·A BeopoulosJean-Marc Nicaud
Nov 2, 2013·Applied Microbiology and Biotechnology·Mingxuan WangYong Q Chen
Jan 22, 2014·Nature Communications·John BlazeckHal S Alper
Mar 4, 2015·Metabolic Engineering·Kangjian QiaoGregory Stephanopoulos
Apr 15, 2015·Bioprocess and Biosystems Engineering·Devyani SalunkeAbhay M Harsulkar
Jun 17, 2015·Applied Microbiology and Biotechnology·Peter GajdošMilan Čertík
Oct 20, 2015·Biotechnology and Bioengineering·Shuyan ZhangChristopher V Rao
Dec 26, 2015·Progress in Lipid Research·Rodrigo Ledesma-Amaro, Jean-Marc Nicaud
Jan 3, 2016·Biotechnology and Bioengineering·Shuangyi BaiJohn Browse
Jan 8, 2016·Nutrients·Je Min LeeWoo Jung Park
Apr 2, 2016·Biotechnology for Biofuels·Jonathan FriedlanderElena E Brevnova
May 24, 2016·Biotechnology for Biofuels·Guokun WangShulin Chen
Jun 21, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jingyang XuGregory Stephanopoulos
Jul 30, 2017·Applied Biochemistry and Biotechnology·Marina Lucía DíazAlicia Carrera