Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis in Yarrowia lipolytica

Frontiers in Microbiology
Feng Xin YanYong Hong Meng

Abstract

Microbial oil triacylglycerol (TAG) from the renewable feedstock attract much attention. The oleaginous yeast Yarrowia lipolytica has become the most studied for lipid biosynthesis. Fatty acid desaturases catalyze the introduction of a double bond into fatty-acid hydrocarbon chains to produce unsaturated fatty acids. Desaturases are known to enhance lipid accumulation. In this study, we have achieved a significant increase in lipid production and increase the unsaturated fatty acids content in Y. lipolytica. By comparing the expression of the native genes of △-9 stearoyl-CoA desaturase (SCD) and △12 desaturase (△12D), and an exogenous △15 desaturase (△15D) from flax in the strain with deleted peroxisomal biogenesis factor 10 (PEX10) and overexpressed diacylglyceride acyl-transferase (DGA1), we found that the strain with overexpressed △15 desaturase accumulated 30.7% lipid. Simultaneously, we explored the effect of two copies of desaturase genes (12D-SCD, 15D-SCD, 12D-15D) on lipid production, and found co-expression of △12D and △15D accumulated 42.6% lipid. The lipid content was further increased by 56.3% through the deletion of the multifunctional enzyme (MFE1) and the overexpression of acetyl-CoA carboxylase (ACC1). Finally, ...Continue Reading

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Citations

Jun 28, 2020·Applied Microbiology and Biotechnology·Ya Dan QianYong Hong Meng
Jan 9, 2021·Bioresource Technology·E Tomás-PejóC González-Fernández

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Methods Mentioned

BETA
PCR
gene knockout
confocal microscopy
fluorescence microscopy
fluorescence assay

Software Mentioned

SPSS
time PCR Miner
Real

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