Overexpression of a citrus basic helix-loop-helix transcription factor (CubHLH1), which is homologous to Arabidopsis activation-tagged bri1 suppressor 1 interacting factor genes, modulates carotenoid metabolism in transgenic tomato

Plant Science : an International Journal of Experimental Plant Biology
Tomoko EndoTakehiko Shimada

Abstract

To explore the transcription factors associated with carotenoid metabolism in citrus fruit, one transcription factor (CubHLH1) was selected through microarray screening in Satsuma mandarin (Citrus unshiu Marc.) fruit, which was treated with exogenous ethylene or gibberellin (GA), accelerating or retarding carotenoid accumulation in peel, respectively. The amino acid sequence of CubHLH1 has homology to Arabidopsis activation-tagged bri1 suppressor 1 (ATBS1) interacting factor (AIF), which is functionally characterized as a negative regulator of the brassinolide (BR) signalling pathway. Yeast two-hybrid analysis revealed that protein for CubHLH1 could interact with Arabidopsis and tomato ATBS1. Overexpression of CubHLH1 caused a dwarf phenotype in transgenic tomato (Solanum lycopersicum L.), suggesting that CubHLH1 has a similar function to Arabidopsis AIF. In the transgenic tomato fruit at ripening stage, the lycopene content was reduced along with the changes in carotenoid biosynthetic gene expression. The abscisic acid (ABA) content of all the transgenic tomato fruit was higher than that of the wild type. These results implied that CubHLH1 is considered to have a similar function to Arabidopsis AIFs and might be directly invol...Continue Reading

References

Nov 28, 2002·Phytochemistry·B Vidya Vardhini, S Seeta Ram Rao
Oct 28, 2004·Journal of Agricultural and Food Chemistry·María-Jesús RodrigoLorenzo Zacarías
Apr 5, 2007·Plant, Cell & Environment·Dong-Hoon JeongGynheung An
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gabriela Toledo-OrtizManuel Rodríguez-Concepción

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Citations

Nov 30, 2019·Plant Science : an International Journal of Experimental Plant Biology·Tianhu Sun, Li Li
Oct 24, 2018·Plant Science : an International Journal of Experimental Plant Biology·Bingcong XingZongsuo Liang

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