Overexpression of a High-Affinity Nitrate Transporter OsNRT2.1 Increases Yield and Manganese Accumulation in Rice Under Alternating Wet and Dry Condition

Frontiers in Plant Science
Bingbing LuoXiaorong Fan

Abstract

Nitrate and manganese (Mn) are necessary elements for the growth and development of rice in paddy soil. Under physiological conditions, we previously reported that the uptake of Mn in roots can be improved by the addition of external nitrate but not ammonium. To investigate the mechanism(s) of this phenotype, we produced plant lines overexpressing OsNRT2.1 and assessed Mn uptake under alternating wet and dry (AWD) and waterlogged (WL) conditions. Under AWD condition, we observed a 31% reduction in grain yields of wild type (WT) plants compared to WL condition. Interestingly, the overexpression of OsNRT2.1 could recover this loss, as OsNRT2.1 transgenic lines displayed higher grain yields than WT plants. We also observed 60% higher grain Mn in the transgenic lines in AWD condition and approximately 30% higher Mn in the grain of transgenic lines in WL condition. We further found that the overexpression of OsNRT2.1 did not alter Mg and Fe in the seeds in either growth condition. The reasons for the increased Mn content in OsNRT2.1 transgenic seeds in AWD condition could be explained by the elevated expression of OsNRAMP family genes including OsNRAMP3, OsNRAMP5, and OsNRAMP6 in node I, the panicle-neck, and the flag leaves. The me...Continue Reading

References

Jun 22, 1999·Current Opinion in Plant Biology·M Stitt
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Shintaro KoikeNaoko K Nishizawa
Jun 1, 2006·Annals of Botany·Xiaolong YanQifa Zhang
Oct 20, 2007·Plant, Cell & Environment·Yi Lin LiQi Rong Shen
Feb 3, 2009·Plant, Cell & Environment·Sichul Lee, Gynheung An
Feb 5, 2010·The Plant Journal : for Cell and Molecular Biology·Yasuhiro IshimaruNaoko K Nishizawa
Sep 22, 2010·Journal of Experimental Botany·Alexander LuxPhilip J White
Nov 3, 2010·The New Phytologist·Bianka SteffensMargret Sauter
Jun 11, 2011·Biological Trace Element Research·Xiaolan GongFashui Hong
Oct 5, 2011·Plant Physiology·Akimasa SasakiJian Feng Ma
Jan 10, 2012·Annual Review of Plant Biology·Guohua XuAnthony J Miller
Oct 13, 2012·BMC Plant Biology·Andrea BorlottiGraziano Zocchi
Sep 21, 2013·Nature Communications·Naoki YamajiJian Feng Ma
Jun 24, 2014·Trends in Plant Science·Naoki Yamaji, Jian Feng Ma
Jun 26, 2014·Journal of Experimental Botany·Meng YangXingming Lian
Mar 10, 2015·Annual Review of Plant Biology·Jian-Ren Shen
Nov 5, 2016·Environmental Geochemistry and Health·Yunyu PanRob N J Comans
Mar 31, 2016·Plant & Cell Physiology·Takatoshi Kiba, Anne Krapp
Apr 22, 2017·Molecular Plant-microbe Interactions : MPMI·Cristina Peris-PerisBlanca San Segundo
May 11, 2017·Plant Physiology·Zhi Chang ChenJian Feng Ma
Dec 20, 2017·Plant Physiology and Biochemistry : PPB·Ambrose Kwaku DziwornuMichael Frei

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Citations

Jan 26, 2020·Scientific Reports·Jagesh Kumar TiwariSwarup Kumar Chakrabarti
Sep 29, 2021·Journal of Integrative Plant Biology·Wei HeFeng Yu

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Methods Mentioned

BETA
transgenic
restriction digest

Software Mentioned

SPSS Statistics

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