Overexpression of a hybrid gene consisting of the amino-terminal fragment of urokinase and carboxyl-terminal domain of bikunin suppresses invasion and migration of human ovarian cancer cells in vitro

International Journal of Cancer. Journal International Du Cancer
Yuji TakeiMitsuaki Suzuki

Abstract

A Kunitz-type protease inhibitor, bikunin, is known to suppress the invasion and metastasis of cancer cells. HI8, a carboxyl-terminal domain of bikunin, is an active site of this glycoprotein. To increase its affinity for cancer cells, we constructed a chimeric gene, ATF-HI8, and investigated the anti-invasive and anti-migratory activity of ATF-HI8 on ovarian cancer cells. ATF-HI8-expressing plasmid and ATF-expressing plasmid were introduced into the highly invasive and metastatic ovarian cancer cell line HRA. The properties of the established cell line (HRA/ATF-HI8) were compared to those of the HRA/ATF and the HRA/luciferase (HRA/LUC, control) cell lines in terms of cell proliferation, invasion and migration. As a result, (i) there were no differences in cell proliferation between HRA/ATF-HI8 and HRA/LUC; (ii) the invasion and migration of HRA/ATF-HI8 cells were significantly inhibited compared to those of HRA/LUC cells; (iii) the migration, but not the invasion, of HRA/ATF cells was significantly inhibited compared to that of HRA/LUC. These results indicate that the overexpression of ATF-HI8 inhibits the invasion and migration of ovarian cancer cells without affecting cell proliferation and suggest that HI8 is involved in th...Continue Reading

References

May 1, 1994·International Journal of Cancer. Journal International Du Cancer·H KobayashiT Terao
Apr 2, 1998·European Journal of Biochemistry·A E Dear, R L Medcalf
Mar 17, 2001·Haemostasis·S P Frostick
Feb 6, 2003·International Journal of Cancer. Journal International Du Cancer·Mika SuzukiToshihiko Terao

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Citations

Nov 13, 2012·The Journal of Surgical Research·Shailendra Kapoor
Jan 12, 2010·International Journal of Cancer. Journal International Du Cancer·Wenjie DongYunlin Wu
Jun 6, 2008·International Journal of Cancer. Journal International Du Cancer·Qiming SunZi-Chun Hua
Mar 19, 2008·Molecular Cancer Therapeutics·Yuji TakeiMitsuaki Suzuki

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