Overexpression of a SDD1-Like Gene From Wild Tomato Decreases Stomatal Density and Enhances Dehydration Avoidance in Arabidopsis and Cultivated Tomato

Frontiers in Plant Science
Samuel Morales-NavarroSimón Ruiz-Lara

Abstract

Stomata are microscopic valves formed by two guard cells flanking a pore, which are located on the epidermis of most aerial plant organs and are used for water and gas exchange between the plant and the atmosphere. The number, size and distribution of stomata are set during development in response to changing environmental conditions, allowing plants to minimize the impact of a stressful environment. In Arabidopsis, STOMATAL DENSITY AND DISTRIBUTION 1 (AtSDD1) negatively regulates stomatal density and optimizes transpiration and water use efficiency (WUE). Despite this, little is known about the function of AtSDD1 orthologs in crop species and their wild stress-tolerant relatives. In this study, SDD1-like from the stress-tolerant wild tomato Solanum chilense (SchSDD1-like) was identified through its close sequence relationship with SDD1-like from Solanum lycopersicum and AtSDD1. Both Solanum SDD1-like transcripts accumulated in high levels in young leaves, suggesting that they play a role in early leaf development. Arabidopsis sdd1-3 plants transformed with SchSDD1-like under a constitutive promoter showed a significant reduction in stomatal leaf density compared with untransformed sdd1-3 plants. Additionally, a leaf dehydratio...Continue Reading

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Citations

May 7, 2019·The New Phytologist·Karl C FetterStephen R Keller
Mar 22, 2019·Frontiers in Plant Science·Lígia T BertolinoJulie E Gray
Mar 19, 2020·Plants·Dimitrios FanourakisGeorgios Tsaniklidis
Dec 18, 2020·Frontiers in Plant Science·Qi YuSuiwen Hou
Oct 25, 2020·Journal of Experimental Botany·Quangang LiuChenghao Li
Dec 9, 2020·Plant Science : an International Journal of Experimental Plant Biology·Ya-Hui WangAi-Sheng Xiong

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Datasets Mentioned

BETA
AT5G15710

Methods Mentioned

BETA
transgenic
electrophoresis
PCR
confocal microscopy
confocal

Software Mentioned

MEGA
BLASTp
Fiji Image J
GraphPad Prism
mPLoc
MUTE
Plant
BioEdit Sequence Alignment Editor
Clustal W
TargetP

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