Overexpression of a SNARE protein AtBS14b alters BR response in Arabidopsis

Botanical Studies
Zhong Xin ZhuDa Nian Yao

Abstract

N-ethyl-maleimide sensitive factor adaptor protein receptor (SNAREs) domain-containing proteins were known as key players in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNAREs in different aspects of plant biology, but the role of many SNAREs are still unknown. In this study, we have characterized the role of Arabidopsis Qc-SNARE protein AtBS14b in brassinosteroids (BRs) signaling pathway. AtBS14b overexpression (AtBS14b ox) plants exhibited short hypocotyl and petioles lengths as well as insensitivity to exogenously supplied BR, while AtBS14b mutants did not show any visible BR-dependent morphological differences. BR biosynthesis enzyme BR6OX2 expression was slightly lower in AtBS14b ox than in wild type plants. Further BR-mediated repression of BR6OX2, CPD and DWF4 was inhibited in AtBS14b ox plants. AtBS14b-mCherry fusion protein localized in vesicular compartments surrounding plasma membrane in N. benthamiana leaves. In addition, isolation of AtBS14b-interacting BR signaling protein, which localized in plasma membrane, showed that AtBS14b directly interacted with membrane steroid binding protein 1 (MSBP1), but did not interact with BAK1 or BRI1. These data suggested that Qc-SNARE prote...Continue Reading

References

Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Daisuke YanoMasao Tasaka
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Steven D. Clouse, Jenneth M. Sasse
May 18, 2007·Annual Review of Cell and Developmental Biology·Volker LipkaRalph Panstruga
Jun 21, 2007·Genes & Development·Niko GeldnerJoanne Chory
Jun 6, 2009·Plant & Cell Physiology·Makoto ShirakawaIkuko Hara-Nishimura
Nov 3, 2009·Plant & Cell Physiology·Kohei HamajiTetsuro Mimura
Nov 26, 2009·Plant Physiology·Andrea BleckmannRüdiger Simon
Jul 24, 2012·The Journal of Biological Chemistry·Toshiyuki OhnishiMasaharu Mizutani

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BETA
GTPases
transgenic
confocal microscopy
two hybrid

Software Mentioned

ImageJ

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