Overexpression of a specific soybean GmGSTU4 isoenzyme improves diphenyl ether and chloroacetanilide herbicide tolerance of transgenic tobacco plants

Journal of Biotechnology
Kostantinos BenekosAthanasios Tsaftaris

Abstract

Plant glutathione transferases (GSTs) superfamily consists of multifunctional enzymes and forms a major part of the plants herbicide detoxification enzyme network. The tau class GST isoenzyme GmGSTU4 from soybean, exhibits catalytic activity towards the diphenyl ether herbicide fluorodifen and is active as glutathione-dependent peroxidase (GPOX). Transgenic tobacco plants of Basmas cultivar were generated via Agrobacterium transformation. The aim was to evaluate in planta, GmGSTU4's role in detoxifying the diphenyl ether herbicides fluorodifen and oxyfluorfen and the chloroacetanilides alachlor and metolachlor. Transgenic tobacco plants were verified by PCR and Southern blot hybridization and expression of GmGSTU4 was determined by RT-PCR. Leaf extracts from transgenic plants showed moderate increase in GST activity towards CDNB and a significant increase towards fluorodifen and alachlor, and at the same time an increased GPOX activity towards cumene hydroperoxide. GmGSTU4 overexpressing plants when treated with 200 μM fluorodifen or oxyfluorfen exhibited reduced relative electrolyte leakage compared to wild type plants. Moreover all GmGSTU4 overexpressing lines exhibited significantly increased tolerance towards alachlor when ...Continue Reading

Citations

Oct 1, 2011·Biotechnology Advances·Parvaiz AhmadF Al-Qurainy
Jan 24, 2015·Current Opinion in Biotechnology·Nikolaos E LabrouEmmanouil Flemetakis
Apr 23, 2016·International Journal of Biological Macromolecules·Katholiki SkopelitouNikolaos E Labrou
Apr 10, 2017·Plant Cell Reports·Irini Nianiou-ObeidatNikolaos E Labrou
Jan 29, 2019·Frontiers in Plant Science·Ágnes GalléPéter Poór
Apr 21, 2020·Frontiers in Plant Science·Xiang-Lin ZhugeHai-Ling Yang
Jul 6, 2017·International Journal of Molecular Sciences·Agnieszka BielachVanesa B Tognetti
Jul 14, 2020·3 Biotech·Swati VaishMahesh Kumar Basantani
Sep 25, 2019·Plants·Sara L MartinTracey James
Nov 22, 2020·International Journal of Molecular Sciences·Mirza HasanuzzamanMasayuki Fujita
Mar 4, 2021·International Journal of Molecular Sciences·Evangelia StavridouIrini Nianiou-Obeidat
Jun 11, 2021·The Science of the Total Environment·Jing Jing Zhang, Hong Yang
Jul 2, 2021·Ecotoxicology and Environmental Safety·Ge-Zi LiGuo-Zhang Kang
Sep 5, 2020·Journal of Agricultural and Food Chemistry·Ke-Liang GuoFei Ye

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.