Overexpression of a virus-like particle influenza vaccine in Eri silkworm pupae, using Autographa californica nuclear polyhedrosis virus and host-range expansion

Archives of Virology
Kenichi MaegawaKuniaki Nerome

Abstract

Ecological investigations of silkworms have revealed that Eri silkworms (Samia cynthia ricini) possess useful morphological and ecological characteristics for virus-like particle (VLP) production, namely non-seasonal breeding, longer lengths, and heavier weights than Bombyx mori silkworms. Furthermore, when vector DNA from Bombyx mori nuclear polyhedrosis virus (BmNPV), which is unable to replicate in Sf9 cells from Eri silkworms, was replaced with the Autographa californica nuclear polyhedrosis virus (AcNPV) vector, three improved AcNPV influenza virus recombinants capable of replication in Sf9 cells were obtained. Although VLP antigens produced previously in silkworms were not evaluated individually, the present recombinant Fukushima (FkH5) and Anhui (AnH7) VLP antigens were detected in tissue fluids and fat bodies of Eri silkworms. Here, we aimed to determine the function of the AcNPV vector and P143 gene by expressing recombinants in Sf9 cells and eri silkworm pupae. The FkH5 recombinant produced high yields of haemagglutinin (HA)-positive VLPs, showing a mean HA titre of 1.2 million. Similarly, high production of H7 HA VLPs was observed in the fat bodies of eri silkworm pupae. Antigenic analysis and electron microscopy exa...Continue Reading

References

Mar 1, 1937·The Journal of Clinical Investigation·J StokesD Shaw
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Elizabeth V L Grgacic, David A Anderson
Jun 14, 2008·The New England Journal of Medicine·Peter F Wright
Jul 31, 1931·The Journal of Experimental Medicine·R E Shope
May 25, 1946·Journal of the American Medical Association·T FRANCISW M BRACE
Aug 6, 2011·Journal of Virological Methods·Vipin Kumar DeoEnoch Y Park
Sep 6, 2012·Applied Microbiology and Biotechnology·Hideki YamajiEiji Konishi
May 29, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Xin WangJinquan Cheng
Mar 24, 2015·Frontiers in Microbiology·Kei-Xian TanLearn-Han Lee

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Citations

Jan 1, 2019·Molecular Biology·B V Syomin, Y V Ilyin

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Methods Mentioned

BETA
transfect
PCR
column filtration

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