Overexpression of aflR Leads to Upregulation of Pathway Gene Transcription and Increased Aflatoxin Production in Aspergillus flavus.

Applied and Environmental Microbiology
J E Flaherty, Gary A Payne

Abstract

The aflatoxin biosynthetic pathway regulatory gene, aflR, encodes a putative 47-kDa protein containing a zinc cluster DNA binding motif. It is required for the transcription of all of the characterized aflatoxin pathway genes in both Aspergillus flavus and Aspergillus parasiticus. The objective of this study was to examine the effects of aflR overexpression on temporal gene expression, aflatoxin production, and nitrate inhibition of aflatoxin biosynthesis in A. flavus. An inducible expression construct was made by fusing the coding region of aflR to the promoter region of the A. flavus adh1 gene. This construct was transformed into A. flavus 656-2 (FGSC A1010), a strain mutated at the aflR locus. Strain 656-2 containing the adh1(p)::aflR construct had induced transcription of two early aflatoxin pathway genes, nor-1 and pksA, and produced wild-type concentrations of aflatoxin in a temporal pattern similar to that of wild-type strains of A. flavus. Strains 656-2 and 86-10 (FGSC A1009) an aflatoxigenic strain, were transformed with a construct containing the constitutive promoter gpdA driving aflR. Transformants of these strains constitutively expressed aflR, fas-1A, pksA, nor-1, and omtA but did not constitutively produce aflato...Continue Reading

References

Jan 1, 1989·Applied and Environmental Microbiology·C P WoloshukC R Adkins
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Apr 30, 1974·Mycopathologia et mycologia applicata·L L Leaich, K E Papa
Aug 1, 1984·Applied and Environmental Microbiology·R L Buchanan, D F Lewis
Jul 1, 1995·Applied and Environmental Microbiology·J E FlahertyC P Woloshuk
Apr 1, 1995·Microbiology·F TrailJ Linz
Jul 1, 1994·Applied and Environmental Microbiology·C P WoloshukG A Payne
Jan 1, 1993·Applied and Environmental Microbiology·G A PayneC P Woloshuk
May 1, 1993·Applied and Environmental Microbiology·C D SkoryJ E Linz
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W BrownT J Leonard

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Citations

Oct 6, 1998·Applied and Environmental Microbiology·B H Liu, F S Chu
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Valdet UkaJosé Diana Di Mavungu
Sep 14, 2007·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·E Afriyie-GyawuT D Phillips
Oct 20, 2012·FEMS Microbiology Reviews·Charles P Woloshuk, Won-Bo Shim
Mar 1, 2007·Mycotoxin Research·M Schmidt-Heydt, R Geisen
Mar 10, 2004·Applied and Environmental Microbiology·Jiujiang YuJoan W Bennett
Mar 12, 2004·Annual Review of Phytopathology·G A Payne, M P Brown
Jan 5, 2006·Applied and Environmental Microbiology·Mihoko TominagaOsamu Akita
Mar 5, 2005·Applied and Environmental Microbiology·Michael J MillerJohn E Linz
Jul 1, 2011·The Journal of Microbiology·Wenbing Yin, Nancy P Keller
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Ana M CalvoNancy P Keller
Mar 27, 2012·Chembiochem : a European Journal of Chemical Biology·Takehito NakazawaKenji Watanabe
May 7, 2004·Applied and Environmental Microbiology·Joseph E Flaherty, Charles P Woloshuk
Sep 6, 2003·Applied and Environmental Microbiology·Joseph E FlahertyCharles P Woloshuk
Jun 19, 2012·Applied Microbiology and Biotechnology·Marzena BlumhoffMichael Sauer
Jun 8, 2018·Frontiers in Microbiology·Qaiser ShakeelLong Yang
Jan 18, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Shu-Yao ZhangDa-Ling Liu
Jul 25, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ghada Ibrahim Ibrahim BarakatAmira Mohammed Sultan
Apr 26, 2011·Annual Review of Phytopathology·Saori Amaike, Nancy P Keller
Feb 3, 2018·The Journal of Microbiology·Firew Tafesse MamoYang Liu
Jul 26, 2007·Journal of Applied Microbiology·F DegolaF M Restivo
Aug 1, 2007·Applied Microbiology and Biotechnology·Perng-Kuang ChangThomas E Cleveland
Mar 4, 2020·Toxins·Isaura CaceresJean-Denis Bailly
Mar 26, 2003·Applied Microbiology and Biotechnology·D BhatnagarT E Cleveland
May 26, 2016·Foodborne Pathogens and Disease·Arianne Costa BaquiãoBenedito Correa
Oct 13, 2016·Annual Review of Genetics·Juliane MacheleidtAxel A Brakhage
May 18, 1999·Fungal Genetics and Biology : FG & B·M P BrownG A Payne
May 29, 2002·Biochimica Et Biophysica Acta·Kenneth C EhrlichPeter J Cotty
Oct 6, 1998·Applied and Environmental Microbiology·D M MeyersG A Payne

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