PMID: 9658130Jul 11, 1998Paper

Overexpression of an alternatively spliced form of c-Myb results in increases in transactivation and transforms avian myelomonoblasts

Journal of Virology
C H WooJ S Lipsick

Abstract

An alternatively spliced form of c-myb exists that encodes an additional 120 amino acids in chicken and 121 amino acids in human and mouse. These amino acids are encoded by an additional exon, termed exon 9A. This exon is not present in v-myb, and proteins containing these amino acids have never been tested for oncogenic transformation. A series of myb constructs was therefore created in order to compare the functions of Myb proteins on the basis of their inclusion or exclusion of the amino acids encoded by exon 9A (E9A). We found that the presence of E9A resulted in a robust increase in transactivation for full-length c-Myb (CCC), as well as the singly truncated derivatives dCC and CCd, while doubly truncated Myb proteins v-Myb (dVd) and dCd did not exhibit any differences in transactivation. The increase in transactivation requires the Myb DNA-binding domain. When the leukemic transformation by the Myb proteins was tested, it was found that cells transformed by dVd resembled monoblasts, while cells transformed by CCC and its derivatives, dCd, dCC, and CCd, resembled myelomonoblasts. Despite differences in the morphology of the hematopoietic cells, the cell surface phenotypes and cell cycle profiles of transformed cells did no...Continue Reading

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Citations

Dec 15, 2000·Oncogene·S HorstmannK H Klempnauer
Mar 3, 2010·Genesis : the Journal of Genetics and Development·Stacey J BakerE Premkumar Reddy
Jan 16, 2008·Molecular and Cellular Biology·John P O'Rourke, Scott A Ness
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Jul 1, 2008·Seminars in Immunology·Kylie T GreigStephen L Nutt

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