PMID: 9192698Jun 24, 1997Paper

Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions

Proceedings of the National Academy of Sciences of the United States of America
N MitsukawaD Shibata

Abstract

A higher plant homologue to the high-affinity phosphate transporter gene of yeast (Saccharomyces cerevisiae) PHO84 was isolated from Arabidopsis thaliana. Expression of the Arabidopsis gene PHT1 at high levels in tobacco-cultured cells increased the rate of phosphate uptake. The uptake activity attributable to the transgene was inhibited by protonophores, suggesting an H+ cotransport mechanism of phosphate uptake, and had a Km of 3.1 microM which is within limits characteristic of high-affinity transport mechanisms. These results indicate that PHT1 encodes a high-affinity phosphate transporter. The transgenic cells exhibited increased biomass production when the supply of phosphate was limited, establishing gene engineering of phosphate transport as one approach toward enhancing plant cell growth.

References

Jun 1, 1990·Applied Microbiology and Biotechnology·R MathumathiK Dharmalingam
Feb 1, 1992·Plant Molecular Biology·Y TakeuchiN T Keen
Jun 1, 1991·Molecular and Cellular Biology·M Bun-YaY Oshima
Jan 1, 1990·Perspectives in Biology and Medicine·C R Honig
Dec 7, 1995·Nature·M J Harrison, M L van Buuren
Oct 1, 1996·The Plant Journal : for Cell and Molecular Biology·Y G LiuR F Whittier
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·U S MuchhalK G Raghothama
Jan 1, 1997·The Plant Journal : for Cell and Molecular Biology·F W SmithE Delhaize
Jan 1, 1995·Plant Physiology·E. Delhaize, P. J. Randall
Jan 1, 1985·Plant Physiology·H Sentenac, C Grignon
Nov 1, 1991·Plant Physiology·Y PoirierJ Schiefelbein
Jan 1, 1984·Planta·C I Ullrich-EberiusA J van Bel
May 1, 1984·Planta
Nov 1, 1984·Planta

❮ Previous
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Citations

Dec 19, 2006·Science in China. Series C, Life Sciences·Feng MingDaleng Shen
Apr 12, 2005·Plant Physiology and Biochemistry : PPB·Matthew M HewittRobert D Slocum
Aug 26, 1999·Trends in Biotechnology·R E Hirsch, M R Sussman
Apr 5, 2001·Current Opinion in Biotechnology·R Hell, H Hillebrand
Oct 25, 2000·Trends in Plant Science·K G Raghothama
Feb 22, 2002·Current Opinion in Plant Biology·Natasha Grotz, Mary Lou Guerinot
Jun 22, 1999·Current Opinion in Plant Biology·F J Maathuis, D Sanders
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Elke RoscheJohn W Patrick
Aug 8, 2002·The Plant Journal : for Cell and Molecular Biology·Stephen R MudgeFrank W Smith
Jun 1, 2004·Nutrition Research Reviews·Paul Christou, Richard M Twyman
Jan 27, 2009·Journal of Experimental Botany·Wei-Yi LinTzyy-Jen Chiou
Mar 23, 2012·Plant & Cell Physiology·Yoichi OgawaDaisuke Shibata
Dec 18, 2001·Plant Physiology·U T MukatiraK G Raghothama
Sep 13, 2002·Plant Physiology·Athikkattuvalasu S KarthikeyanKashchandra G Raghothama
Mar 27, 2009·Plant Physiology·Yoshiharu SawakiHiroyuki Koyama
May 28, 2011·Plant Physiology·Carlos Calderón-VázquezLuis Herrera-Estrella
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·K. G. Raghothama
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Arthur Grossman, Hideki Takahashi
Aug 29, 2013·BioMed Research International·Keisuke MatsuiYoshikazu Tanaka
Jan 1, 2002·The Arabidopsis Book·Yves Poirier, Marcel Bucher
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·U S Muchhal, K G Raghothama
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·W T LauE K O'Shea
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Uta PaszkowskiSteven P Briggs
May 16, 2002·Physiologia Plantarum·Steffen AbelCarla A Delatorre
Sep 23, 2014·Trends in Plant Science·Robert MeisterDaniel P Schachtman
Aug 18, 2012·Cellular and Molecular Life Sciences : CMLS·Ajay JainKashchandra G Raghothama

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