Overexpression of angiopoietin-1 increases CD133+/c-kit+ cells and reduces myocardial apoptosis in db/db mouse infarcted hearts.

PloS One
Heng ZengJian-Xiong Chen

Abstract

Hematopoietic progenitor CD133(+)/c-kit(+) cells have been shown to be involved in myocardial healing following myocardial infarction (MI). Previously we demonstrated that angiopoietin-1(Ang-1) is beneficial in the repair of diabetic infarcted hearts. We now investigate whether Ang-1 affects CD133(+)/c-kit(+) cell recruitment to the infarcted myocardium thereby mediating cardiac repair in type II (db/db) diabetic mice. db/db mice were administered either adenovirus Ang-1 (Ad-Ang-1) or Ad-β-gal systemically immediately after ligation of the left anterior descending coronary artery (LAD). Overexpression of Ang-1 resulted in a significant increase in CXCR-4/SDF-1α expression and promoted CD133(+)/c-kit(+), CD133(+)/CXCR-4(+) and CD133(+)/SDF-1α(+) cell recruitment into ischemic hearts. Overexpression of Ang-1 led to significant increases in number of CD31(+) and smooth muscle-like cells and VEGF expression in bone marrow (BM). This was accompanied by significant decreases in cardiac apoptosis and fibrosis and an increase in myocardial capillary density. Ang-1 also upregulated Jagged-1, Notch3 and apelin expression followed by increases in arteriole formation in the infarcted myocardium. Furthermore, overexpression of Ang-1 resulte...Continue Reading

Associated Clinical Trials

Oct 21, 2013·Dr. Antonio Pellicer Martínez

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Citations

Sep 24, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xiao-Hua YuChao-Ke Tang
Aug 21, 2012·Trends in Cardiovascular Medicine·William HiesingerY Joseph Woo
Oct 15, 2014·Journal of Cellular and Molecular Medicine·Xuwei HouJian-Xiong Chen
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Dec 28, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lele WuLanfang Li
Jan 31, 2017·International Journal of Cardiology·Yong-Kang TaoJian-Xiong Chen
Mar 8, 2017·Journal of Cellular and Molecular Medicine·Shuo WangJian-Xiong Chen
Nov 10, 2017·Stem Cells Translational Medicine·Maritza E MayorgaFeng Dong
Feb 1, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Laura E Crotty AlexanderEllen C Breen
Mar 29, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Kiave Yune Howangyin, Jean-Sébastien Silvestre
Mar 29, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jessie S JeonRoger D Kamm
Dec 24, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Heng ZengJian-Xiong Chen
Jul 4, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Lanfang LiJian-Xiong Chen
May 6, 2021·International Journal of Molecular Sciences·Vinoth Kumar RethineswaranSang-Mo Kwon

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Methods Mentioned

BETA
SMA
transfection

Software Mentioned

NIH Image
TINA
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