Overexpression of Arabidopsis miR157b induces bushy architecture and delayed phase transition in Torenia fournieri.

Planta
Masahito ShikataNorihiro Ohtsubo

Abstract

miR156/157 is a small RNA molecule that is highly conserved among various plant species. Overexpression of miR156/157 has been reported to induce bushy architecture and delayed phase transition in several plant species. To investigate the effect of miR157 overexpression in a horticultural plant, and to explore the applicability of miRNA to molecular breeding, we introduced Arabidopsis MIR157b (AtMIR157b) into torenia (Torenia fournieri). The resulting 35S:AtMIR157b plants showed a high degree of branching along with small leaves, which resembled miR156/157-overexpressing plants of other species. We also isolated torenia SBP-box genes with target miR156/157 sequences and confirmed that their expression was selectively downregulated in 35S:AtMIR157b plants. The reduced accumulation of mRNA was probably due to sequence specificity. Moreover, expression of torenia homologs of the SBP-box protein-regulated genes TfLFY and TfMIR172 was also reduced by AtmiR157 overexpression. These findings suggest that the molecular mechanisms of miR156/157 regulation are conserved between Arabidopsis and torenia. The bushy architecture and small leaves of 35S:AtMIR157b torenia plants could be applied in molecular breeding of various...Continue Reading

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Citations

Aug 19, 2014·Journal of Experimental Botany·Elena A VidalRodrigo A Gutiérrez
Mar 5, 2015·Molecular Plant·Sachin Teotia, Guiliang Tang
Sep 1, 2015·Frontiers in Plant Science·Giao N NguyenSurya Kant
Jul 16, 2013·Plant Science : an International Journal of Experimental Plant Biology·Jeffrey J FischerDouglas G Muench
Oct 30, 2014·Frontiers in Plant Science·Madelaine E Bartlett, Beth Thompson
Sep 17, 2015·Essays in Biochemistry·Ianis G Matsoukas
Dec 11, 2019·International Journal of Molecular Sciences·Chenfei ZhengRongling Wu
Sep 19, 2020·Plants·John Martin Jerome JeyakumarMuthu Thiruvengadam
Apr 17, 2020·International Journal of Molecular Sciences·Ali Anwar, Ju-Kon Kim
Jan 16, 2020·International Journal of Molecular Sciences·Huai-Xia ZhangZhen-Hui Gong
Mar 17, 2020·Frontiers in Plant Science·Huai-Xia ZhangZhen-Hui Gong

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