Overexpression of Arabidopsis thaliana brassinosteroid-related acyltransferase 1 gene induces brassinosteroid-deficient phenotypes in creeping bentgrass

PloS One
Yun-Jeong HanJeong-Il Kim

Abstract

Brassinosteroids (BRs) are naturally occurring steroidal hormones that play diverse roles in various processes during plant growth and development. Thus, genetic manipulation of endogenous BR levels might offer a way of improving the agronomic traits of crops, including plant architecture and stress tolerance. In this study, we produced transgenic creeping bentgrass (Agrostis stolonifera L.) overexpressing a BR-inactivating enzyme, Arabidopsis thaliana BR-related acyltransferase 1 (AtBAT1), which is known to catalyze the conversion of BR intermediates to inactive acylated conjugates. After putative transgenic plants were selected using herbicide resistance assay, genomic integration of the AtBAT1 gene was confirmed by genomic PCR and Southern blot analysis, and transgene expression was validated by northern blot analysis. The transgenic creeping bentgrass plants exhibited BR-deficient phenotypes, including reduced plant height with shortened internodes (i.e., semi-dwarf), reduced leaf growth rates with short, wide, and thick architecture, high chlorophyll contents, decreased numbers of vascular bundles, and large lamina joint bending angles (i.e., erect leaves). Subsequent analyses showed that the transgenic plants had signific...Continue Reading

References

Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·M M NeffJ Chory
Jun 5, 2002·The Plant Cell·Gerard J Bishop, Csaba Koncz
Sep 25, 2003·Annual Review of Plant Biology·Shozo Fujioka, Takao Yokota
Dec 17, 2003·Journal of Plant Growth Regulation·Priti Krishna
Apr 15, 2004·Current Opinion in Biotechnology·Tomoaki Sakamoto, Makoto Matsuoka
Feb 4, 2005·Journal of Experimental Botany·Masanobu NakamuraAkira Nagatani
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Naoki TakahashiMinami Matsui
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Edward M TurkMichael M Neff
Apr 12, 2005·Plant Biology·C Müssig
Oct 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brigitte PoppenbergerDianna Bowles
Apr 18, 2006·Current Opinion in Plant Biology·John C D'Auria
Jul 29, 2008·Biochemical and Biophysical Research Communications·Bo Kyung KimSunghwa Choe
Aug 19, 2008·The Plant Cell·Chuan-yin WuRoger I Pennell
Nov 18, 2008·Plant Physiology and Biochemistry : PPB·Andrzej Bajguz, Shamsul Hayat
Jul 28, 2009·New Biotechnology·Uday K Divi, Priti Krishna
Mar 3, 2010·Annual Review of Plant Biology·Tae-Wuk Kim, Zhi-Yong Wang
Jul 30, 2010·Plant Molecular Biology·Leeann E ThorntonMichael M Neff
Mar 25, 2011·BMC Plant Biology·Sigrid HusarBrigitte Poppenberger
Mar 23, 2012·The Plant Cell·Cécile VrietChristophe Reuzeau
May 2, 2012·Journal of Experimental Botany·Ulrich Kutschera, Zhi-Yong Wang
Jun 29, 2012·Trends in Plant Science·Sikander Pal ChoudharyLam-Son Phan Tran
Sep 12, 2012·Journal of Integrative Plant Biology·Baolin Zhao, Jia Li
Oct 2, 2012·The Plant Journal : for Cell and Molecular Biology·Sunhwa ChoiIldoo Hwang
Oct 17, 2012·PloS One·Katja SchneiderKeiko Sugimoto
Mar 28, 2013·Development·Jia-Ying ZhuZhi-Yong Wang
Mar 29, 2014·Plant Cell Reports·Cui ZhangKang Chong
Nov 19, 2014·BMC Plant Biology·Florian SchröderCarsten Müssig
Jan 24, 2015·The New Phytologist·Youssef Belkhadir, Yvon Jaillais
Feb 13, 2015·Journal of Experimental Botany·Amar Pal Singh, Sigal Savaldi-Goldstein
May 15, 2015·Plant Biotechnology Journal·Uday K DiviPriti Krishna
Nov 20, 2015·Frontiers in Plant Science·Shivani SainiPratap Kumar Pati
Jan 1, 2013·Plants·Tadashi MatsumotoKeimei Oh

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
X-ray
glucosylation

Software Mentioned

ImageJ
SPSS statistics

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.