Overexpression of Aurora-A kinase promotes tumor cell proliferation and inhibits apoptosis in esophageal squamous cell carcinoma cell line

Cell Research
Xiaoxia WangQimin Zhan

Abstract

Aurora-A kinase, a serine/threonine protein kinase, is a potential oncogene. Amplification and overexpression of Aurora-A have been found in several types of human tumors, including esophageal squamous cell carcinoma (ESCC). It has been demonstrated that cells overexpressing Aurora-A are more resistant to cisplatin-induced apoptosis. However, the molecular mechanisms mediating these effects remain largely unknown. In this report, we showed that overexpression of Aurora-A through stable transfection of pEGFP-Aurora-A in human ESCC KYSE150 cells significantly promoted cell proliferation and inhibited cisplatin- or UV irradiation-induced apoptosis. Cleavages of caspase-3 and poly (ADP-ribose) polymerase (PARP) in Aurora-A overexpressing cells were substantially reduced after cisplatin or UV treatment. Furthermore, we found that silencing of endogenous Aurora-A kinase with siRNA substantially enhanced sensitivity to cisplatin- or UV-induced apoptosis in human ESCC EC9706 cells. In parallel, overexpression of Aurora-A potently upregulated the expression of Bcl-2. Moreover, the knockdown of Bcl-2 by siRNA abrogated the Aurora-A's effect on inhibiting apoptosis. Taken together, these data provide evidence that Aurora-A overexpression ...Continue Reading

References

Oct 18, 1996·Cell·E S AlnemriJ Yuan
Jun 17, 1998·Genes & Development·V Cryns, J Yuan
Sep 30, 1998·Cell·D R Green
Jun 8, 1999·Annual Review of Immunology·J C Rathmell, C B Thompson
Feb 13, 2001·Biochemical and Biophysical Research Communications·S Y SunG S Wu
Jun 23, 2001·BMJ : British Medical Journal·J Sjöström, J Bergh
May 11, 2002·Nature Reviews. Cancer·Frederik H Igney, Peter H Krammer
Jun 26, 2003·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·T NoguchiW Müller
Jun 15, 2004·Cancer Letters·Hiroaki ToshimitsuKohsuke Sasaki
Oct 8, 2004·The Journal of Biological Chemistry·Qiyuan LiuJin Q Cheng
Nov 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tong TongQimin Zhan
Mar 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eiji TanakaYutaka Shimada
Jul 1, 2005·Molecular and Cellular Biology·Chang-Tze Ricky YuChi-Ying F Huang

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Citations

Jun 24, 2009·Nature Reviews. Molecular Cell Biology·Andrew J Holland, Don W Cleveland
Nov 28, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yan WangGong Yang
Apr 25, 2012·The Journal of Cell Biology·Akihito InokoMasaki Inagaki
Apr 2, 2009·Expert Opinion on Investigational Drugs·Chun Hei Antonio CheungJang-Yang Chang
Jun 4, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hong-Chun LiuChang-Lian Zhu
Jan 1, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ning WuZhiming Chen
Jul 6, 2010·Acta histochemica·Yu LeiHan Rui-Fa
Nov 27, 2015·Biochemical and Biophysical Research Communications·Se-Ryeon KimSang-Beom Seo
Nov 26, 2008·Cancer Letters·Lili QinQimin Zhan
Aug 19, 2009·Biochemical Pharmacology·Minglun LiClaus Belka
Apr 10, 2012·Biochemical and Biophysical Research Communications·Ning YinQimin Zhan
Dec 14, 2011·Cancer Biology & Therapy·Lorna Jane WarnockJo Milner
Apr 30, 2016·Acta Biochimica Et Biophysica Sinica·Xiaoxia WangBinquan Wang
Jun 17, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul M BarrJonathan W Friedberg
Dec 22, 2009·Molecular BioSystems·Ran Brosh, Varda Rotter
May 1, 2014·Molecular Informatics·Mohammad Neaz Morshed
Aug 7, 2018·Anti-cancer Drugs·Juan Liu, Cheng-Yong Qin
Jul 19, 2006·Genes, Chromosomes & Cancer·Laura T SmithCarl Morrison
Mar 28, 2008·Expert Review of Anticancer Therapy·Pol M Specenier, Jan B Vermorken
Nov 30, 2016·Oncotarget·Callum McKenzie, Pier Paolo D'Avino
Aug 24, 2010·Radiation Research·Eun-Taex OhHeon Joo Park

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