Overexpression of Bcl-2 attenuates MPP+, but not 6-ODHA, induced cell death in a dopaminergic neuronal cell line

Neurobiology of Disease
Y J OhK L O'Malley

Abstract

In order to investigate the role of Bcl-2 in dopaminergic cells, we established a dopaminergic neuronal cell line (MN9D) stably expressing human Bcl-2 (MN9D/Bcl-2) or neomycin (MN9D/Neo). Overexpression of Bcl-2 in MN9D cells attenuated cell death due to treatment of mitochondrial electron transport inhibitors including N-methyl-4-phenylpyridinium, whereas it did not prevent cell death induced by reagents generating reactive oxygen species including 6-hydroxy-dopamine. Moreover, the rate of glucose uptake in MN9D/Bcl-2 was significantly lower than that in MN9D/Neo after MPP+ treatment. Thus, Bcl-2 may counter aberrations in mitochondrial electron transfer processes by altering energy metabolism within the MN9D cells.

Citations

Mar 10, 2005·The Journal of Biological Chemistry·Jason CallioCharleen T Chu
Jan 8, 2011·Molecular Neurodegeneration·Alison I BernsteinKaren L O'Malley
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·D OffenO Bernard
Dec 13, 2005·Journal of Neurochemistry·Armando P SignoreJun Chen
Feb 25, 2003·The Journal of Biological Chemistry·William Andrew Holtz, Karen Laurel O'Malley
Jul 10, 2012·Neuromolecular Medicine·Björn SpittauKerstin Krieglstein
Aug 30, 2002·Neurotoxicology and Teratology·A Nicotra, S Parvez

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