Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells

Experimental Cell Research
Weibiao HuangTimothy A Miller

Abstract

Bone morphogenetic proteins (BMP) play a pivotal role in growth and differentiation of osteoblastic lineage cells. BMPs are potent stimulators of bone formation in various animal models. To understand the mechanism of BMP action in bone cells, we have investigated the effects of overexpression of the BMP-2 gene on proliferation and differentiation of UMR-106 rat osteosarcoma cells. A stable UMR-106 cell line overexpressing the BMP-2 gene was established by transfection of cells using a mammalian expression vector harboring human BMP-2 cDNA followed by G418 selection. After introduction of the BMP-2 gene, UMR-106 cells appeared more spindle-shaped in morphology compared to the predominantly cuboidal appearance of the parental cells. Overexpression of BMP-2 markedly inhibited proliferation as measured by cell counting and [3H]thymidine incorporation assays. Extracellular matrix (ECM) derived from cells overexpressing BMP-2 exhibited a less supportive effect on proliferation of UMR cells than did ECM derived from parental cells. Furthermore, cell-cell communication through gap junctions was reduced more than 50% as determined by nondisruptive fluorescent dye transfer assays. Overexpression of BMP-2 significantly stimulated express...Continue Reading

Citations

May 16, 2008·Journal of Cell Communication and Signaling·Tsuyoshi ShimoAkira Sasaki
Jun 6, 2008·Arthritis Research & Therapy·Francois N K KwongChristopher H Evans
Oct 8, 2011·Bioscience, Biotechnology, and Biochemistry·Ryohei KonoHirotoshi Utsunomiya
Sep 12, 2014·International Orthopaedics·Alan NguyenAaron W James
May 13, 2009·Journal of Zhejiang University. Science. B·Jin-jing WangHong Bu
Aug 26, 2003·Molecular Cancer·Martin P KracklauerGuido M Sclabas
Apr 4, 2008·Regulatory Peptides·Maria Petersson
Dec 23, 2003·Journal of Cellular Biochemistry·K D BrubakerR L Vessella
Apr 19, 2016·Nutrition Research and Practice·Mi Hwa ParkMihyang Kim
Jul 12, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Cynthia A LuppenBaruch Frenkel
Apr 20, 2006·Journal of Bone and Mineral Metabolism·Chie WadaToshihiko Nagata
Dec 2, 2005·American Journal of Physiology. Cell Physiology·Zhongyong LiHenry J Donahue
May 21, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alexandra StanleyEileen M Shore
Apr 26, 2020·The Journal of Biological Chemistry·Amel DudakovicAndre J van Wijnen
May 15, 2020·Genome Génome / Conseil National De Recherches Canada·Siavash Salek ArdestaniYounes Miar
Aug 15, 2015·Spine·Branko SkovrljSamuel K Cho
Apr 23, 2015·American Journal of Medical Genetics. Part a·Asmaa S Abu Maziad, Laurie H Seaver
Jan 9, 2007·Molecular and Cellular Biochemistry·Ling WuWeidong Tian
Dec 1, 2010·Clinical Reviews in Bone and Mineral Metabolism·Shane A J Lloyd, Henry J Donahue
Dec 20, 2005·The Journal of Surgical Research·Jason RoostaeianTimothy A Miller
Feb 28, 2006·Cancer Letters·Isaac Yi Kim, Seong Jin Kim
Nov 21, 2007·Biomaterials·Marie-Eve MarquisNathalie Faucheux

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