Overexpression of c-myc induces apoptosis at the prophase of meiosis of rat primary spermatocytes

Molecular Reproduction and Development
K KodairaM Ueda

Abstract

Transgenic rats expressing the rat c-myc gene under the control of the human metallothionein II A promoter were produced. We found that the female transgenic rats were fertile, but that the male transgenic rats were sterile. Atrophy of the seminiferous tubules and depletion of sperm were observed in the sterile male testes. The expression of differential stage-specific mRNAs, including those of the c-kit receptor proto-oncogene, meiotic heat-shock protein 70 gene, acrosin gene, and transition protein 1 gene, was analyzed by the reverse transcriptase-polymerase chain reaction during spermatogenesis. The results suggested that spermatogenesis in these sterile rats were arrested at the prophase of meiosis in the primary spermatocytes. We found that apoptotic DNA fragmentation occurred in primary spermatocytes of the sterile transgenic rats. These results suggest that overexpression of the c-myc gene induces apoptosis at the prophase meiosis of the primary spermatocytes thereby causing male sterility in the c-myc transgenic rats.

References

Apr 3, 1992·Cell·G I EvanD C Hancock
Nov 30, 1990·Biochemical and Biophysical Research Communications·S KashiwabaraT Baba
Oct 8, 1991·Biochimica Et Biophysica Acta·U KlemmW Engel
Jan 1, 1989·Molecular Reproduction and Development·P C YelickN B Hecht
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A E Griep, H Westphal
Aug 25, 1987·Nucleic Acids Research·K HayashiK Yoneda
Dec 3, 1993·Cell·M RassoulzadeganF Cuzin
Jan 1, 1995·Developmental Genetics·N B Hecht
Sep 1, 1994·Proceedings of the Society for Experimental Biology and Medicine·A BartkeD Turyn
Mar 1, 1994·Molecular Reproduction and Development·T NinomiyaA Yuki
Feb 1, 1993·Current Opinion in Genetics & Development·G I Evan, T D Littlewood

❮ Previous
Next ❯

Citations

May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C G Print, K L Loveland
Jul 12, 2002·Molecular Reproduction and Development·Masumi HirabayashiShinichi Hochi
Oct 26, 2005·Transgenic Research·Laurent TessonIgnacio Anegon
Nov 7, 1998·Biochimica Et Biophysica Acta·D BaytelJ Don
Jul 14, 2001·Molecular Biology of the Cell·M T HemannC W Greider
Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Y Matsui
Aug 8, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Heikki T TurunenMatti Poutanen
Dec 5, 2000·Anatomia, histologia, embryologia·Y Kon, D Endoh
May 26, 2004·Biochemical and Biophysical Research Communications·Shang-Feng LiuZhao Wang
Jan 9, 2003·The Urologic Clinics of North America·Edward D KimRandall B Meacham
Dec 13, 2005·Journal of Biochemistry and Molecular Biology·Shang-feng LiuZhao Wang
Dec 31, 2002·Molecular Reproduction and Development·Yuka NamikiYasuhiro Kon
Sep 4, 2009·Acta Biochimica Et Biophysica Sinica·Shuying DongSongbin Fu
Aug 4, 2007·Molecular and Cellular Biochemistry·Ming-Chao XieZhao Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis