Overexpression of CaHSP26 in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance

Journal of Plant Physiology
Shangjing GuoQingwei Meng

Abstract

A sweet pepper cDNA clone, CaHSP26 encoding the chloroplast (CP)-localized small heat shock protein (sHSP), was isolated and characterized with regard to its sequence, response to various temperatures and function in transgenic tobacco plants. The deduced amino acid sequence contained three highly conserved regions, showing high identities with other plant sHSPs. Expression of the CaHSP26 gene showed that the mRNA accumulation of CaHSP26 was induced by heat stress. Higher transcript levels were observed when sweet pepper leaves were treated at 42 degrees C for 3h. However, the expression of the CaHSP26 gene was not induced by chilling stress (4 degrees C) in the absence of heat shock (HS). But the transcripts were still detected at 48h at 4 degrees C after HS while not at 25 degrees C. The photochemical efficiency of PSII (Fv/Fm) and the oxidizable P700 in transgenic tobacco overexpressing CaHSP26 were higher than that in wild type tobacco during chilling stress under low irradiance. These results suggest that CP sHSP protein plays an important role in protection of PSII and PSI during chilling stress under low irradiance.

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Citations

Mar 29, 2008·Cell Stress & Chaperones·Masood SiddiqueKlaus-Dieter Scharf
Oct 13, 2014·Plant Physiology and Biochemistry : PPB·Ismayil S ZulfugarovChoon-Hwan Lee
Feb 11, 2012·Journal of Plant Physiology·Jie ZouXinbo Chen
May 8, 2007·Current Opinion in Plant Biology·Sachin KotakKlaus-Dieter Scharf
Sep 19, 2016·Biochemical and Biophysical Research Communications·Yan ZhangJian Hua
Dec 7, 2017·Plant, Cell & Environment·Norio Murata, Yoshitaka Nishiyama
Dec 5, 2018·Frontiers in Plant Science·Fengfeng DangShuilin He
Jun 27, 2019·International Journal of Molecular Sciences·Tzu-Hsing KoTzan-Chain Lee
Mar 12, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Radhouane Chaffai, Ameur Cherif

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