Overexpression of calreticulin modulates protein kinase B/Akt signaling to promote apoptosis during cardiac differentiation of cardiomyoblast H9c2 cells.

The Journal of Biological Chemistry
Kan KageyamaTakahito Kondo

Abstract

Calreticulin is a Ca(2+)-binding molecular chaperone of the lumen of the endoplasmic reticulum. Calreticulin has been shown to be essential for cardiac and neural development in mice, but the mechanism by which it functions in cell differentiation is not fully understood. To examine the role of calreticulin in cardiac differentiation, the calreticulin gene was introduced into rat cardiomyoblast H9c2 cells, and the effect of calreticulin overexpression on cardiac differentiation was examined. Upon culture in a differentiation medium containing fetal calf serum (1%) and retinoic acid (10 nm), cells transfected with the calreticulin gene were highly susceptible to apoptosis compared with controls. In the gene-transfected cells, protein kinase B/Akt signaling was significantly suppressed during differentiation. Furthermore, protein phosphatase 2A, a Ser/Thr protein phosphatase, was significantly up-regulated, implying suppression of Akt signaling due to dephosphorylation of Akt by the up-regulated protein phosphatase 2A via regulation of Ca(2+) homeostasis. Thus, overexpression of calreticulin promotes differentiation-dependent apoptosis in H9c2 cells by suppressing the Akt signaling pathway. These findings indicate a novel mechani...Continue Reading

References

Mar 15, 1976·Experimental Cell Research·B W Kimes, B L Brandt
Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Aug 1, 1992·The Biochemical Journal·M MichalakM Opas
Jul 23, 1991·Biochimica Et Biophysica Acta·Y KitagawaM Nagao
Nov 1, 1995·Trends in Biochemical Sciences·Z Songyang, L C Cantley
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·M AndjelkovićB A Hemmings
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·H KonishiU Kikkawa
Feb 21, 1997·Cell·K H Krause, M Michalak
Aug 29, 1998·The Journal of Cell Biology·L M JohnP Camacho
Oct 28, 1998·Nature·M J BerridgeP Lipp
Mar 23, 1999·The Journal of Cell Biology·N MesaeliM Michalak
May 14, 1999·Trends in Biochemical Sciences·T A MillwardB A Hemmings
Mar 31, 2000·Experimental Cell Research·F RauchR St-Arnaud
Aug 23, 2000·The Journal of Cell Biology·K NakamuraM Michalak
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S SatoT Tsuruo
Sep 23, 2000·Nature·D Srivastava, E N Olson
Jan 10, 2001·The Journal of Cell Biology·J M HolaskaB M Paschal
Apr 18, 2001·Trends in Cell Biology·S JohnsonP Eggleton
May 23, 2001·The Journal of Biological Chemistry·M P FadelM Opas

❮ Previous
Next ❯

Citations

Feb 9, 2013·Pathology Oncology Research : POR·Mohammadreza ZamanianRozita Rosli
Mar 16, 2005·Biochemical and Biophysical Research Communications·Yoshito IharaTakahito Kondo
Mar 20, 2004·Pharmacology & Therapeutics·Amere Subbarao Sreedhar, Peter Csermely
Nov 26, 2002·Biochimica Et Biophysica Acta·Marek MichalakMichal Opas
Sep 22, 2006·Antioxidants & Redox Signaling·Cristen PantanoYvonne M W Janssen-Heininger
Jul 14, 2010·Antioxidants & Redox Signaling·Maaike C W van den Berg, Boudewijn M T Burgering
Feb 10, 2004·Molecular Biology of the Cell·Nasrin Mesaeli, Clark Phillipson
Jul 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Manuel A PalleroJoanne E Murphy-Ullrich
Jun 25, 2009·Anti-cancer Drugs·Yong Kui ZhangDong Mei Diao
Jul 12, 2011·Physiological Reviews·Mark A SussmanMichael McGregor
Jul 31, 2004·American Journal of Respiratory Cell and Molecular Biology·Heiko A GolponNorbert F Voelkel
Jul 15, 2006·Breast Cancer Research : BCR·Thea PugatschRonen Beeri
Oct 12, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Osamu SuzukiJunichiro Matsuda
May 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Thu H Truong, Kate S Carroll
Dec 5, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wenjuan YaoWei Zhang
Sep 8, 2010·Differentiation; Research in Biological Diversity·Viviana di GiacomoAmelia Cataldi
Jan 10, 2009·Brain Research·Traci L PawlowskiMatthew J Huentelman
Aug 16, 2008·Brain Research·Chang Hwan ParkGyeong Jae Cho
Oct 5, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Xiaochu ZhangMichal Opas
May 8, 2007·Molecular Genetics and Metabolism·Kiyoko HattoriFumio Endo
May 18, 2007·Molecular and Cellular Endocrinology·Sung-E ChoiYup Kang
Nov 12, 2009·BioFactors·Ilse VandecaetsbeekPeter Vangheluwe
May 19, 2012·PloS One·Eric D RogersJennifer V Schmidt
Jul 20, 2007·Reproductive Sciences·Kurt Meyer, Lubo Zhang
Oct 7, 2003·Oncogene·Tiziana ToniniPier Paolo Claudio
Jul 25, 2015·Journal of Cellular Physiology·Maria Angela D'amicoAngela Di Baldassarre
Jul 11, 2006·American Journal of Physiology. Cell Physiology·Carmen C SucharovLeslie Leinwand
Sep 1, 2006·The Journal of Biological Chemistry·Yasushi HayashidaYoshito Ihara
Aug 13, 2002·The Journal of Biological Chemistry·Chandrahasa R YellaturuGadiparthi N Rao

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.