PMID: 9657259Jul 10, 1998Paper

Overexpression of cardiotrophin-1 and gp130 during experimental acute Chagasic cardiomyopathy

Immunology Letters
B ChandrasekarG L Freeman

Abstract

Cardiotrophin-1 (CT-1), a cytokine with structural similarities to interleukin-6, has been shown to signal through gp130-dependent pathways. In vitro, CT-1 promotes the survival and induces hypertrophy of neonatal cardiomyocytes. Since acute Chagas' disease involves an inflammatory response followed by chamber dilation, with subsequent compensatory hypertrophy, we hypothesized CT-1 and gp130 may participate in this disease process. Thus, we investigated expression and localization of these moieties during acute Chagasic cardiomyopathy. Lewis rats (n = 6/group) were either inoculated with cell culture-derived T. cruzi trypomastigotes or saline, and sacrificed 15 days later. Hearts were collected for histology, immunohistochemistry (IHC), mRNA, and protein analyses. Histology showed dense myocardial infection with amastigotes and diffuse mononuclear cell infiltrate. Northern blot analysis showed low level expression of CT-1 mRNA in controls, which was markedly elevated in infected animals (2.5-fold; P < 0.001). Similarly, Western blotting showed a twofold elevation of CT-1 protein in infected animals (P < 0.025). Likewise, levels of both gp130 mRNA and protein were low in controls, but were approximately threefold higher in infec...Continue Reading

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Citations

Jul 23, 2003·Infection and Immunity·Christine A Petersen, Barbara A Burleigh
Sep 17, 2008·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·David Stejskal, Viktor Ruzicka
Jul 11, 2000·Clinical and Experimental Immunology·B ChandrasekarG L Freeman
Dec 26, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·H Mongue-DinY Fromes
Jul 7, 2020·Frontiers in Cellular and Infection Microbiology·Lindice M NisimuraDaniel Adesse
Apr 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·M OkunoK Yamauchi-Takihara
Dec 3, 1999·International Journal of Experimental Pathology·D S Latchman
Feb 16, 2000·Pharmacology & Therapeutics·D S Latchman
Jun 30, 2021·Molecular Microbiology·Felipe S CoelhoAngela H Lopes

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