Overexpression of caveolin-1 induces alteration of multidrug resistance in Hs578T breast adenocarcinoma cells

International Journal of Cancer. Journal International Du Cancer
Chuanxi Cai, Jianwen Chen

Abstract

Caveolin-1 is a major caveolae-coat protein involved in a variety of cell signaling processes. Some studies have suggested that the level of caveolin-1 expression positively correlates with multi-drug resistance in cancer cells. We demonstrated for the first time that Hs578T doxorubicin resistant cells (Hs578T/Doxo), which contain low levels of endogenous caveolin-1 and high levels of P-glycoprotein, are rendered drug-sensitive by overexpression of exogenous caveolin-1. MTT assays showed that after overexpressing caveolin-1, the drug resistance of Hs578T/Doxo cells to doxorubicin and cisplatin was reduced from 25.4 +/- 1.5 and 65.3 +/- 2.5 microg/ml to 0.8 +/- 0.15 and 23.2 +/- 2.1 microg/ml, respectively (i.e. reduced by 97% and 64%, respectively). Furthermore, using rhodamine-123 efflux assays, we observed a significant decrease in P-glycoprotein activity in caveolin-1 overexpressing cells, similar to that observed with 5 microM cyclosporine A or 10 microM verapamil, 2 inhibitors of P-glycoprotein activity. Using confocal microscopy, subcellular fractionation and co-immunoprecipitation assays, a possible physical interaction between caveolin-1 and P-glycoprotein in the caveolae membrane was observed in Hs578T/Doxo cells overe...Continue Reading

References

Feb 21, 1992·Cell·K G RothbergR G Anderson
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J SmartR G Anderson
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·M MurataK Simons
Jan 1, 1995·Molecular Membrane Biology·M P LisantiM Sargiacomo
Jun 30, 1995·The Journal of Biological Chemistry·S LiM P Lisanti
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·P E SchererM P Lisanti
May 17, 1996·The Journal of Biological Chemistry·C MineoR G Anderson
Nov 15, 1996·The Journal of Biological Chemistry·E J SmartR G Anderson
Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Nov 26, 1998·The Journal of Biological Chemistry·Y LavieM Liscovitch
Nov 26, 1999·Biochemical Pharmacology·O JonssonK Grankvist
Feb 21, 2002·Biochimica Et Biophysica Acta·Julie JodoinRichard Beliveau
Feb 28, 2002·American Journal of Clinical Pathology·Ben DavidsonReuven Reich
Mar 30, 2002·Advanced Drug Delivery Reviews·Terry R Stouch, Olafur Gudmundsson
Jul 26, 2002·The Journal of Biological Chemistry·Vimal A PatelAndrey Sorokin
Apr 25, 2003·International Journal of Cancer. Journal International Du Cancer·Graham P PidgeonKenneth V Honn

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Citations

Sep 9, 2011·Breast Cancer Research and Treatment·Neill PataniMitch Dowsett
Feb 5, 2016·Scientific Reports·Chih-Yuan LeeJin-Shing Chen
Jul 2, 2010·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Delphine BeghinSophie Gil
Apr 7, 2009·Cancer Letters·Massimo Serra, Katia Scotlandi
May 25, 2005·Fundamental & Clinical Pharmacology·Andrew OwenDavid J Back
Mar 1, 2007·Journal of Neurochemistry·Stéphane BarakatRichard Béliveau
Mar 9, 2010·International Journal of Cancer. Journal International Du Cancer·Clotilde Ferrándiz-HuertasAntonio Ferrer-Montiel
May 17, 2008·Cancer Letters·Elke BurgermeisterMatthias P A Ebert
Jul 9, 2004·Biochemical and Biophysical Research Communications·Chuanxi CaiJianwen Chen
Jun 1, 2020·Cancer Metastasis Reviews·Vibha Singh, Christophe Lamaze
Oct 31, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Sumadi Lukman AnwarSamuel J Haryono
Apr 25, 2020·The Journal of Cell Biology·Robert G PartonNicholas Ariotti
Oct 3, 2015·Oncotarget·Zhiyu WangHonglin Situ
May 27, 2017·Oxidative Medicine and Cellular Longevity·Shengqi WangZhiyu Wang
Dec 28, 2019·International Journal of Molecular Sciences·Ilja L Kruglikov, Philipp E Scherer

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