Overexpression of CDC14B causes mitotic arrest and inhibits zygotic genome activation in mouse preimplantation embryos.

Cell Cycle
Mariano G BuffoneRichard M Schultz

Abstract

Following fertilization the transition from a highly differentiated oocyte to a totipotent 2-cell embryo requires two unique mitotic cell cycles. The first cell cycle is characterized by a prolonged G(1) phase, DNA replication (S phase) that occurs separately in the female and male pronuclei, and a short G(2) phase that occur in the absence of cell growth. During the second cell cycle, G(1) is short whereas G(2) is prolonged and occurs concurrently with zygotic genome activation, which is essential for progression past the 2-cell stage. CDC14B, a dual specificity phosphatase that counteracts cyclin dependent kinase 1 (CDK1/CDC2A) action, regulates mitosis in somatic cells and prevents premature meiotic resumption in mouse oocytes. It is not known if CDC14B plays a role during the unique mitotic cell cycles of preimplantation development. We report that CDC14B is present in mouse embryos and localizes to mitotic centrosomes and spindles. Overexpressing CDC14B in 1-cell embryos results in 40% and 60% of the embryos arresting at the 1- and 2-cell stages, respectively. Embryos arrested at the 1-cell stage contained reduced CDC2A activity, whereas embryos arrested at the 2-cell stage were in G(2) and failed to activate the zygotic g...Continue Reading

Citations

Dec 24, 2010·Development·Aurélie ClémentKathleen L Gould
Oct 3, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Aurélie ClémentKathleen L Gould
Jul 15, 2015·Clinical and Experimental Reproductive Medicine·Hee Jung KangDong-Wook Park
May 27, 2017·Human Fertility : Journal of the British Fertility Society·Yadava Bapurao JeveTarek Gelbaya
Jun 28, 2018·Biology Open·Leif R NeitzelEthan Lee
Dec 19, 2017·The Journal of Reproduction and Development·Ying LinLi Chen

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