PMID: 9164985May 1, 1997Paper

Overexpression of cellular glutathione peroxidase does not affect expression of plasma glutathione peroxidase or phospholipid hydroperoxide glutathione peroxidase in mice offered diets adequate or deficient in selenium

The Journal of Nutrition
W H ChengX G Lei

Abstract

Selenium-dependent cellular glutathione peroxidase (GPX1) overexpressing [GPX1(+)] mice were derived by microinjecting a 5.3-kb cloned entire mouse GPX1 genomic DNA into fertilized eggs. The objective of this study was to determine the effect of GPX1 overexpression and dietary selenium on the expression of selenoperoxidases and the status of lipid peroxidation of these transgenic animals. An experiment with a 2 x 2 factorial arrangement of treatments with 15 GPX1(+) and 15 control mice (2 mo old) was conducted for 8 wk. Ten mice of each group (half males and females) were fed a Se-deficient, Torula yeast basal diet (0.02 mg Se/kg, no supplemental vitamin E) and five mice (three males and two females) were fed the basal diet supplemented with 0.51 mg Se/kg as Na2SeO3. The GPX1(+) mice had greater GPX1 activities (one- to sixfold, P < 0.0001) than the control mice at both levels of dietary selenium in all tissues except for liver, in which such difference (100%, P < 0.05) was observed only in Se-deficient mice. The GPX1 mRNA level in kidney and in lung of the Se-deficient GPX1(+) mice was 81% and 7.5-fold greater (P < 0.003) than the respective control level. Overexpression of GPX1 did not alter phospholipid hydroperoxide glutath...Continue Reading

References

Jun 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R F Burk
Dec 15, 1986·Biochemical Pharmacology·S D Mercurio, G F Combs
Jun 10, 1988·Nucleic Acids Research·Y S HoJ D Crapo
Mar 29, 1985·Biochimica Et Biophysica Acta·F UrsiniC Gregolin
Feb 9, 1973·Science·J T RotruckW G Hoekstra
Oct 20, 1995·Archives of Biochemistry and Biophysics·M J KelnerG T Mullenbach
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·S C VendelandP D Whanger
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·O MirochnitchenkoM Inouye
Jul 1, 1995·The Journal of Nutritional Biochemistry·Merrill J. ChristensenChristian D. Wray

❮ Previous
Next ❯

Citations

Mar 25, 2005·Journal of Animal Physiology and Animal Nutrition·A S MüllerJ Pallauf
Sep 24, 2005·The Journal of Nutrition·Xin Gen Lei, Wen-Hsing Cheng
Jun 18, 2005·Molecular & Cellular Proteomics : MCP·Linghua QiuJohn J Kopchick
Aug 22, 2008·Human & Experimental Toxicology·Ss YalçinM Ozdemir
Jun 29, 2010·Antioxidants & Redox Signaling·Matthew P PepperXin Gen Lei
Jul 25, 2019·Clinical and Experimental Pharmacology & Physiology·Duc Toan PhamHyoung-Chun Kim
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·N WeissJ Loscalzo
Jul 14, 2010·Antioxidants & Redox Signaling·Xin Gen Lei, Marko Z Vatamaniuk
Jul 27, 2014·The Journal of Biological Chemistry·Sumangala P ShettyPaul R Copeland
Feb 7, 2016·Clinical and Experimental Pharmacology & Physiology·Huynh Nhu MaiHyoung-Chun Kim
Jul 2, 2003·Neurotoxicity Research·B H Juurlink
Dec 19, 2015·Physiological Reviews·Xin Gen LeiElias S J Arnér
Oct 24, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J N KellerT S Kindy
Feb 22, 2013·Circulation·Sanjana DayalSteven R Lentz
May 1, 1999·Applied and Environmental Microbiology·Y HanX G Lei
May 26, 2018·Free Radical Biology & Medicine·Jia-Qiang HuangXin Gen Lei
Jun 4, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Naveen SharmaHyoung-Chun Kim
Dec 8, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E ZoidisK Feggeros
Mar 23, 2011·Free Radical Biology & Medicine·Shi Kui WangXin Gen Lei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.