Overexpression of centrosomal protein Nlp confers breast carcinoma resistance to paclitaxel

Cancer Biology & Therapy
Weihong ZhaoQimin Zhan

Abstract

Nlp (ninein-like protein), an important molecule involved in centrosome maturation and spindle formation, plays an important role in tumorigenesis and its abnormal expression was recently observed in human breast and lung cancers. In this study, the correlation between overexpression of Nlp and paclitaxel chemosensitivity was investigated to explore the mechanisms of resistance to paclitaxel and to understand the effect of Nlp upon apoptosis induced by chemotherapeutic agents. Nlp expression vector was stably transfected into breast cancer MCF-7 cells. With Nlp overexpression, the survival rates, cell cycle distributions and apoptosis were analyzed in transfected MCF-7 cells by MTT test and FCM approach. The immunofluorescent assay was employed to detect the changes of microtubule after paclitaxel treatment. Immunoblotting analysis was used to examine expression of centrosomal proteins and apoptosis associated proteins. Subsequently, Nlp expression was retrospectively examined with 55 breast cancer samples derived from paclitaxel treated patients. Interestingly, the survival rates of MCF-7 cells with Nlp overexpressing were higher than that of control after paclitaxel treatment. Nlp overexpression promoted G2-M arrest and atten...Continue Reading

References

Oct 16, 1999·Biology of the Cell·J L SalisburyS L Barrett
Sep 27, 2000·Current Topics in Developmental Biology·W L Lingle, J L Salisbury
Oct 18, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·R Krishna, L D Mayer
Apr 28, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·A C DingemansG Giaccone
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Wilma L LingleJeffrey L Salisbury
May 29, 2003·Current Cancer Drug Targets·M AbalI Barasoain
Jun 5, 2003·Medical Hypotheses·Q Kong
Jun 20, 2003·Molecular Pharmacology·Cristiano FerliniGiovanni Scambia
Mar 3, 2004·Molecular Interventions·Stéphanie Dutertre, Claude Prigent
Nov 18, 2004·Journal of the National Cancer Institute·Richard D KennedyD Paul Harkin
Jun 14, 2005·Biochimica Et Biophysica Acta·Yaqing Wang, Fernando Cabral
Aug 9, 2006·International Journal of Cancer. Journal International Du Cancer·Hua YangJin Q Cheng
Oct 6, 2006·Cancer Chemotherapy and Pharmacology·Pascal SèveJohn R Mackey
May 22, 2007·Molecular Cancer Therapeutics·Lourdes A GomezCarlos Perez-Stable
Oct 28, 2008·Neoplasia : an International Journal for Oncology Research·Louis CheslerWilliam A Weiss
Dec 23, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·E A EisenhauerJ Verweij
Jan 22, 2009·Cancer Metastasis Reviews·Susana A GodinhoDavid Pellman
Jun 11, 2009·The Journal of Biological Chemistry·Shunqian JinQimin Zhan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.