PMID: 7581303Jan 1, 1995Paper

Overexpression of choline acetyltransferase reconstitutes discrete acetylcholine release in some but not all synapse formation-defective neuroblastoma cells

Journal of Physiology, Paris
Z G ZhongH Higashida

Abstract

Secretion of acetylcholine (ACh) in neuroblastoma cells overexpressing choline acetyltransferase (ChAT) was examined. With transient transfection of ChAT cDNA, neuroblastoma cells, which have no endogenous ChAT and either adhere to myotubes or not, failed to form functional synapses, and thus no evidence for release of ACh was detected. Stable neuroblastoma cell lines overexpressing ChAT accumulated ACh inside the cell, and slowly released ACh to the outside of the cell in a calcium-independent fashion. However, after co-culturing them with rat muscle cells, these transformed cells adhered to myotubes and ACh was secreted in a discrete fashion into the synaptic cleft efficiently in some neuroblastoma cell lines but rather inefficiently in another cell line. The results show that the latent secretion machinery of ChAT overexpressing neuroblastoma cells either is competent or possess defect(s) in ACh release.

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D G Puro, M Nirenberg
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P NelsonM Nirenberg
Nov 1, 1990·Brain Research. Developmental Brain Research·P E SpoerriF J Roisen
Jul 1, 1991·Journal of Neurochemistry·J L McManaman, F G Crawford
Jan 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J B OlmstedJ Rosenbaum
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·M NirenbergH Fukui
Feb 1, 1982·Cancer Genetics and Cytogenetics·K Kano-TanakaT Tanaka
Jul 30, 1982·Science·R T BartusA S Lippa
Jan 1, 1995·Journal of Physiology, Paris·Y Dunant, M Israël
Jun 1, 1993·Trends in Neurosciences·M E Hasselmo, J M Bower
May 1, 1994·Brain Research. Molecular Brain Research·C Quirin-StrickerM Schmitt
May 1, 1994·Journal of Neurochemistry·D Wu, L B Hersh
Apr 1, 1993·Journal of Neurochemistry·H MisawaT Deguchi

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Citations

Apr 29, 1998·Molecular Neurobiology·M Israël, Y Dunant
Jan 1, 1995·Journal of Physiology, Paris·Y Dunant, M Israël
Jan 5, 2002·Journal of Physiology, Paris·Xavier CastellVladimír Dolezal

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