PMID: 7541536Jul 3, 1995Paper

Overexpression of csk inhibits acid-induced activation of NHE-3

Proceedings of the National Academy of Sciences of the United States of America
Y YamajiR J Alpern

Abstract

Opossum kidney OKP cells express an apical membrane Na+/H+ antiporter that is encoded by NHE-3 (for Na+/H+ exchanger 3) and is similar in many respects to the renal proximal tubule apical membrane Na+/H+ antiporter. Chronic incubation of OKP cells in acid medium for 24 hr increases Na+/H(+)-antiporter activity and NHE-3 mRNA abundance. The increase in Na+/H(+)-antiporter activity was not prevented by H7, a protein kinase C/protein kinase A inhibitor, but was prevented by herbimycin A, a tyrosine kinase inhibitor. Incubation of cells in acid medium increased c-src activity, and this was inhibited by herbimycin A. To determine the role of the src family of nonreceptor protein-tyrosine kinases, Csk (for carboxyl-terminal src kinase), a physiologic inhibitor of these kinases, was overexpressed in OKP cells. In three clones overexpressing csk, acid-induced increases in Na+/H(+)-antiporter activity and NHE-3 mRNA abundance were inhibited. In these clones, inhibition of acid activation of Na+/H(+)-antiporter activity paralleled inhibition of acid activation of c-src. Neither herbimycin A nor overexpression of csk inhibited dexamethasone-induced increases in Na+/H(+)-antiporter activity. These studies show that decreases in pH activate...Continue Reading

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Citations

Jun 7, 1996·Biochimica Et Biophysica Acta·M T Brown, J A Cooper
Aug 5, 1998·Kidney International·H TsuganezawaR J Alpern
Jun 26, 2002·Kidney International·Hirohiko TsuganezawaRobert J Alpern
Mar 15, 1996·The Journal of Clinical Investigation·T S ChuR J Alpern
Dec 16, 2004·The Journal of Clinical Investigation·Shaoying LiRobert J Alpern
Dec 16, 2004·The Journal of Clinical Investigation·Stephen L Gluck
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yuehan ZhouWalter F Boron
Oct 31, 2006·Seminars in Nephrology·I Alexandru Bobulescu, Orson W Moe
Nov 14, 2006·Experimental Cell Research·Claudiu Iordache, Marek Duszyk
May 9, 2012·The International Journal of Biochemistry & Cell Biology·Oliver OehlkeEleni Roussa
Jan 12, 2016·PloS One·Hyeon-Ju LeeBermseok Oh
Jul 21, 2017·Circulation Journal : Official Journal of the Japanese Circulation Society·Sung-Moon KimBermseok Oh
Aug 18, 2006·Journal of the American Society of Nephrology : JASN·Walter F Boron
Sep 12, 2019·Physiological Reviews·S F Pedersen, L Counillon
May 3, 1996·The Journal of Biological Chemistry·R A Kandasamy, J Orlowski
Mar 20, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M W MuschE B Chang
Dec 28, 2002·Annals of the New York Academy of Sciences·Hisayoshi HayashiSergio Grinstein
Jul 9, 1998·The American Journal of Physiology·R C FitzgeraldG Triadafilopoulos
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Doris Joy D EspirituJose A L Arruda
Aug 10, 2007·American Journal of Physiology. Renal Physiology·Xiaojing YangPatricia A Preisig
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alvaro LucioniEugene B Chang
Jun 13, 2002·American Journal of Physiology. Renal Physiology·Pedro GomesPatrício Soares-da-Silva
Oct 12, 1999·The American Journal of Physiology·O S RuizJ A Arruda

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