Overexpression of cyclin D1 induces the reprogramming of differentiated epidermal cells into stem cell-like cells

Cell Cycle
Along ZhaoXiaobing Fu

Abstract

It has been reported that Wnt/β-catenin is critical for dedifferentiation of differentiated epidermal cells. Cyclin D1 (CCND1) is a β-catenin target gene. In this study, we provide evidence that overexpression of CCND1 induces reprogramming of epidermal cells into stem cell-like cells. After introducing CCND1 gene into differentiated epidermal cells, we found that the large flat-shaped cells with a small nuclear-cytoplasmic ratio changed into small round-shaped cells with a large nuclear-cytoplasmic ratio. The expressions of CK10, β1-integrin, Oct4 and Nanog in CCND1 induced cells were remarkably higher than those in the control group (P < 0.01). In addition, the induced cells exhibited a high colony-forming ability and a long-term proliferative potential. When the induced cells were implanted into a wound of laboratory animal model, the wound healing was accelerated. These results suggested that overexpression of CCND1 induced the reprogramming of differentiated epidermal cells into stem cell-like cells. This study may also offer a new approach to yield epidermal stem cells for wound repair and regeneration.

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Citations

Jul 5, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Fangfang WangXiaobing Fu
Nov 6, 2018·BMC Genomics·Juan Carlos Higareda-AlmarazMarcel Scheideler
Sep 14, 2017·Molecular Medicine Reports·Shiqi SheYing-Jie Wang
Mar 21, 2018·Neural Regeneration Research·Jian WengBao-Guo Jiang
Jan 9, 2021·Stem Cell Research & Therapy·Xiaohong ZhaoRixing Zhan
Oct 4, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Ting WangNongyue He

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Methods Mentioned

BETA
transfection
PCR
fluorescence microscopy
transgenic
electrophoresis
light scattering
biopsy
biopsies

Software Mentioned

SPSS

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