Overexpression of cypin alters dendrite morphology, single neuron activity, and network properties via distinct mechanisms

Journal of Neural Engineering
Ana R RodríguezB L Firestein

Abstract

This study investigates the effect that overexpression of cytosolic PSD-95 interactor (cypin), a regulator of synaptic PSD-95 protein localization and a core regulator of dendrite branching, exerts on the electrical activity of rat hippocampal neurons and networks. We cultured rat hippocampal neurons and used lipid-mediated transfection and lentiviral gene transfer to achieve high levels of cypin or cypin mutant (cypinΔPDZ; PSD-95 non-binding) expression cellularly and network-wide, respectively. Our analysis revealed that although overexpression of cypin and cypinΔPDZ increase dendrite numbers and decrease spine density, cypin and cypinΔPDZ distinctly regulate neuronal activity. At the single cell level, cypin promotes decreases in bursting activity while cypinΔPDZ reduces sEPSC frequency and further decreases bursting compared to cypin. At the network level, by using the Fano factor as a measure of spike count variability, cypin overexpression results in an increase in variability of spike count, and this effect is abolished when cypin cannot bind PSD-95. This variability is also dependent on baseline activity levels and on mean spike rate over time. Finally, our spike sorting data show that overexpression of cypin results in...Continue Reading

References

Apr 9, 1999·Nature Neuroscience·J M IlardiZ H Sheng
Dec 14, 1999·Neuron·B L FiresteinD S Bredt
Feb 13, 2001·Journal of Neurophysiology·P VetterM Häusser
Feb 17, 2001·Current Opinion in Neurobiology·H T Cline
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·M Sheng
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric SchnellRoger A Nicoll
Oct 3, 2002·Nature Reviews. Neuroscience·Rachel O L Wong, Anirvan Ghosh
Oct 15, 2003·Neuron·David S Bredt, Roger A Nicoll
Jan 20, 2004·Nature Neuroscience·Barbara F AkumBonnie L Firestein
Jul 2, 2004·Neural Computation·R Quian QuirogaY Ben-Shaul
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pratima ThakurJens Rettig
Aug 27, 2005·Molecular Biology of the Cell·Maxine ChenBonnie L Firestein
Feb 10, 2006·Journal of Neurophysiology·Carl GoldGyörgy Buzsáki
Oct 6, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erik I CharychBonnie L Firestein
Dec 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jean-Claude BéïqueRichard L Huganir
Feb 19, 2008·Journal of Neuroscience Methods·Nadav Raichman, Eshel Ben-Jacob
Oct 24, 2008·Frontiers in Molecular Neuroscience·Dove Keith, Alaa El-Husseini
Nov 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jiaping GuJames Q Zheng
Dec 5, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xindao HuErik W Dent
Jan 17, 2009·Neuron·Jacek JaworskiCasper C Hoogenraad
Mar 20, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Carsten K PfefferChristian A Hübner
May 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Peter J UhlhaasEugenio Rodriguez
Oct 2, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Gregory Z Tau, Bradley S Peterson
Jan 19, 2010·Trends in Cognitive Sciences·Peter J UhlhaasWolf Singer
Feb 23, 2010·Nature Neuroscience·Mark M ChurchlandKrishna V Shenoy
Apr 27, 2010·Journal of Neuroscience Methods·Uri T Eden, Mark A Kramer
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Naoya TakahashiYuji Ikegaya
Aug 6, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christopher G LanghammerBonnie L Firestein
Nov 30, 2010·Journal of Visualized Experiments : JoVE·Melinda K KutzingBonnie L Firestein
Dec 18, 2010·Neural Computation·Chris Christodoulou, Aristodemos Cleanthous
Apr 15, 2011·Critical Reviews in Biomedical Engineering·Yoonkey Nam, Bruce C Wheeler
Dec 29, 2011·Annals of Biomedical Engineering·Melinda K KutzingBonnie L Firestein
Apr 3, 2012·Molecular and Cellular Neurosciences·Vaishali A Kulkarni, Bonnie L Firestein
Jun 23, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dion K DickmanGraeme W Davis
Jun 23, 2012·Frontiers in Computational Neuroscience·Fikret E KapucuJari A K Hyttinen
Aug 17, 2012·Journal of Neurophysiology·Benjamin WhiteJózsef Fiser

❮ Previous
Next ❯

Citations

Jul 22, 2018·Neurobiology of Disease·Przemyslaw SwiatkowskiBonnie L Firestein
Jul 1, 2021·ACS Chemical Biology·Marcela S BucardoYitzhak Tor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain-Computer Interface

A brain-computer interface, also known as a brain-machine interface, is a bi-directional communication pathway between an external device and a wired brain. Here is the latest research on this topic.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Erik I CharychB L Firestein
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Chia-Yi Tseng, B L Firestein
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Hongxin Chen, B L Firestein
© 2022 Meta ULC. All rights reserved