Overexpression of D-amino acid oxidase from Bradyrhizobium japonicum, enhances resistance to glyphosate in Arabidopsis thaliana

Plant Cell Reports
Hongjuan HanQuan-Hong Yao

Abstract

The glyphosate resistance in Escherichia coli and Arabidopsis was due to D-amino acid oxidase expression. Transgenic glyphosate-resistant crops have a high percentage in the total area devoted to transgenic crops worldwide. D-amino acid oxidase (DAAO) can metabolize glyphosate by oxidative cleavage of the carbon-nitrogen bond on the carboxyl side and yield aminomethyl phosphonic acid and glyoxylate, which are less toxic to plants than glyphosate. To date, reports on the use of DAAO to enhance glyphosate resistance in plants are lacking. In this paper, we report synthesis, and codon usage optimization for plant expression, of the DAAO gene by successive polymerase chain reaction from Bradyrhizobium japonicum. To confirm the glyphosate resistance of the DAAO gene, the recombinant plasmid pYPX251 (GenBank Accession No: AY178046) harboring the wild-type DAAO gene was transformed into DH5α. The positive transformants grew well both on solid and in liquid M9 medium containing 200 mM glyphosate. The optimized DAAO gene was transformed into Arabidopsis and 9 days after application of 10 mM glyphosate, the 4-week-old wild-type plants all died; by contrast, transgenic plants could grow normally. The proline content and peroxidase activit...Continue Reading

References

Jan 25, 1989·Nucleic Acids Research·E E MurrayM Eberle
Dec 18, 2001·The Journal of Biological Chemistry·Viviana JobLoredano Pollegioni
Dec 28, 2002·Journal of Agricultural and Food Chemistry·Stephen O DukeReid J Smeda
Mar 26, 2003·European Journal of Biochemistry·Gianluca MollaLoredano Pollegioni
Apr 24, 2004·The Journal of Biological Chemistry·Mario MörtlLoredano Pollegioni
May 25, 2004·Science·Linda A CastleMichael W Lassner
Sep 30, 2006·Applied Microbiology and Biotechnology·Ai-Sheng XiongQuan-Hong Yao
Feb 3, 2007·The Journal of Biological Chemistry·Daniel L SiehlRobert J Keenan
Mar 31, 2007·Cellular and Molecular Life Sciences : CMLS·L PollegioniG Molla
Dec 15, 2007·Pest Management Science·Gerald M DillStephen R Padgette
Feb 15, 2008·Pest Management Science·Stephen O Duke, Stephen B Powles
Jan 27, 2009·Journal of Plant Physiology·Yong XueQuanhong Yao
Oct 30, 2009·The Journal of Biological Chemistry·Mattia PedottiLoredano Pollegioni
Jun 10, 2010·Nature Biotechnology·Emily Waltz
Oct 6, 2010·Journal of Agricultural and Food Chemistry·Stephen O Duke
Mar 15, 2011·Trends in Biotechnology·Loredano Pollegioni, Gianluca Molla
Jun 15, 2011·The FEBS Journal·Loredano PollegioniDaniel Siehl

❮ Previous
Next ❯

Citations

Apr 3, 2021·Current Microbiology·Keren Hernández GuijarroLeonardo Erijman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nihon hōigaku zasshi = The Japanese journal of legal medicine
M KageukaS Kashimura
Advances in Enzymology and Related Areas of Molecular Biology
K Yagi
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
K Yagi, M Nishikimi
Biochimica Et Biophysica Acta
A Baich, D J Pierson
© 2021 Meta ULC. All rights reserved