Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer

Scientific Reports
Huayue LinZhong-Ying Zhang

Abstract

Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed DHX32 contributes to CRC remains largely unknown. In this study, we reported that DHX32 was overexpressed in human colon cancer cells. Overexpressed DHX32 promoted SW480 cancer cells proliferation, migration, and invasion, as well as decreased the susceptibility to chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed that depleting DHX32 in SW480 colon cancer cells suppressed expression of WISP1, MMP7 and VEGFA in the Wnt pathway, and anti-apoptotic gene BCL2 and CA9, however, elevated expression of pro-apoptotic gene ACSL5. The findings suggested that overexpressed DHX32 played an important role in CRC progression and metastasis and that DHX32 has the potential to serve as a biomarker and a novel therapeutic target for CRC.

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Citations

Jan 27, 2017·Journal of the National Cancer Institute·Wanpei CaiAlan Prem Kumar
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ailian Zhang, Jincheng Li
Jan 18, 2019·Journal of Translational Medicine·Yi LiuXueqiong Zhu
Mar 31, 2017·Oncology Letters·Meng WangXixiong Kang
Mar 24, 2021·Technology in Cancer Research & Treatment·Bei LiTingbao Zhao

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Methods Mentioned

BETA
PCR
Transfection
ELISA
Assay
flow cytometry

Software Mentioned

RT 2 Profiler PCR Array Data Analysis Template

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