Overexpression of DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) And DNA (Cytosine-5)-Methyltransferase 3A (DNMT3A) Is Associated with Aggressive Behavior and Hypermethylation of Tumor Suppressor Genes in Human Pituitary Adenomas

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Hou-Shi MaXu-Hui Li

Abstract

BACKGROUND Alteration of DNA methylation of tumor suppressor genes (TSGs) is one of the most consistent epigenetic changes in human cancers. DNMTs play several important roles in DNA methylation and development of cancers. Regarding DNMTs protein expressions, little is known about the clinical significance and correlation with promoter methylation status of TSGs in human pituitary adenomas. MATERIAL AND METHODS We analyzed the protein expression of 3 DNMTs using immunohistochemistry and assessed DNA hypermethylation of RASSF1A, CDH13, CDH1, and CDKN2A (p16) in 63 pituitary adenomas. We examined associations between DNMTs expression and clinicopathological features or promoter methylation status of TSGs. RESULTS Overexpression of DNMTs was detected in pituitary adenomas. Frequencies of DNMT1 overexpression were significantly higher in macroadenomas, invasive tumors, and grade III and IV tumors. DNMT3A was frequently detected in invasive tumors and grade IV tumors. In addition, DNMT1 and DNMT3A were frequently detected in high-methylation tumors. Furthermore, in multivariate logistic regression, the significant association between DNMT1 or DNMT3A and high-methylation status persisted after adjusting for clinicopathological featur...Continue Reading

Citations

Dec 21, 2019·Pituitary·C HageR Salvatori
Apr 2, 2020·The Journal of Clinical Endocrinology and Metabolism·Borbála SzabóHenriett Butz
Mar 24, 2020·Endocrine Reviews·Vinaya Srirangam Nadhamuni, Márta Korbonits
Jun 6, 2020·Biomedicines·Wilhelm GossingLars Radke
May 30, 2019·Frontiers in Endocrinology·Blake M HauserIan F Dunn
Dec 4, 2019·Expert Review of Endocrinology & Metabolism·Angeline Jia Jia ShenChristopher J Yates
Nov 19, 2020·Pituitary·Zhenqing WeiRenzhi Wang

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Methods Mentioned

BETA
PCR
electrophoresis
surgical resection

Software Mentioned

SAS

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