PMID: 10760816Apr 13, 2000Paper

Overexpression of epidermal growth factor receptor in human head and neck squamous carcinoma cell lines correlates with matrix metalloproteinase-9 expression and in vitro invasion

International Journal of Cancer. Journal International Du Cancer
P O-CharoenratS Eccles

Abstract

Numerous reports have shown an association between overexpression of the epidermal growth factor receptor (EGFR), and poor prognosis in head and neck squamous cell carcinomas (HNSCC), however, the underlying mechanisms are still unclear. In the present study, we set out to determine whether EGFR expression was associated with in vitro invasive capacity in a panel of four established and ten newly derived HNSCC lines. Ten of the cell lines expressed high levels of EGFR as determined by a ligand-binding assay and dot blot analysis, whereas the remaining four showed weak overexpression or normal levels of EGFR. The ability of cells to invade through Matrigel was found to be higher in the EGFR overexpressing cell lines (p < 0. 0001). Expression levels of matrix metalloproteinases (MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, MMP-13, MT1-MMP) and tissue inhibitors of MMP (TIMP-1, TIMP-2) were evaluated by semiquantitative RT-PCR, substrate zymography and western blot. We found a strong positive correlation between EGFR levels and the expression of MMP-9 mRNA (r(2) = 0.95; p < 0.0001), MMP-9 enzyme activity (r(2) = 0.8099; p < 0.0001) and an inverse correlation with TIMP-1 (r(2) = 0.48; p = 0.0059). In six selected HNSCC lines,...Continue Reading

References

Feb 1, 1986·British Journal of Cancer·G P CowleyB A Gusterson
Jan 1, 1995·Analytical Biochemistry·P Fernández-ResaA R Quesada
May 1, 1994·Analytical Biochemistry·D E Kleiner, W G Stetler-Stevenson
Jan 21, 1993·The New England Journal of Medicine·E E VokesW K Hong
Apr 1, 1996·Journal of Cellular Physiology·G M GrantR J Klebe
Feb 14, 1998·International Journal of Cancer. Journal International Du Cancer·H ModjtahediC Dean
Jun 13, 1998·Journal of the National Cancer Institute·J Rubin GrandisD J Tweardy
Mar 30, 1999·International Journal of Cancer. Journal International Du Cancer·G AlmadoriF Ottaviani
Jan 6, 2000·Oral Oncology·G T ThomasP M Speight

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Citations

Dec 15, 2010·International Journal of Molecular Sciences·Limei YanMasao Iwamori
Jul 7, 2011·Indian Journal of Clinical Biochemistry : IJCB·Ragini D SinghPrabhudas S Patel
Nov 24, 2011·Rheumatology·Nia Emami-Shahri, Thorsten Hagemann
May 19, 2006·Expert Opinion on Biological Therapy·Sabrina OliveiraRaymond M Schiffelers
Oct 20, 2006·Expert Opinion on Biological Therapy·Dominique ArseneJean-Marie Reimund
Sep 2, 2015·Oncology Reports·Dan-Dan DongGao Li
Sep 10, 2014·Cancer Cell International·Paulette M TamashiroToshihiko Kawamori
Apr 30, 2010·The Journal of Investigative Dermatology·Jennifer FreytagPaul J Higgins
Mar 7, 2003·Clinical Colorectal Cancer·Roger B Cohen
May 7, 2008·Biochemical and Biophysical Research Communications·Sariya Nuchanardpanit SinpitaksakulPrasit Pavasant
Jun 6, 2015·Archives of Pathology & Laboratory Medicine·Lizandra JimenezJeffrey E Segall
Jun 16, 2004·International Journal of Cancer. Journal International Du Cancer·Pornchai O-charoenratSuzanne A Eccles
Jun 14, 2005·Head & Neck·Gina M S Howell, Jennifer R Grandis
Jan 25, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K AsanoH Suzaki
Aug 5, 2003·The Laryngoscope·Eben L RosenthalCandece L Gladson
Jun 15, 2006·International Journal of Urology : Official Journal of the Japanese Urological Association·Jong-Shiaw JinWei-Hwa Lee
Feb 22, 2012·International Journal of Laboratory Hematology·J WelshL A Madden
Jul 17, 2004·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·P A BrennanD Górecki
Mar 2, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·S C Lim
Feb 18, 2003·Otolaryngologic Clinics of North America·Patrick K Ha, Joseph A Califano
Oct 16, 2009·The International Journal of Biochemistry & Cell Biology·Wei-Guo HuangZhi-Qiang Xiao
Jul 19, 2002·International Journal of Cancer. Journal International Du Cancer·Pornchai O-CharoenratSuzanne Eccles
Jun 8, 2001·International Journal of Cancer. Journal International Du Cancer·C SugimotoS Matsukawa
Oct 22, 2016·Experimental Cell Research·Jyotsna NambiarBipin G Nair
May 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ezra E W CohenEverett E Vokes
Jul 16, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·John Mendelsohn, Jose Baselga
Sep 11, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul M HarariJames A Bonner
Apr 25, 2006·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Jong-Shiaw JinChien-Yu Bai
Nov 10, 2000·International Journal of Cancer. Journal International Du Cancer·P O-CharoenratS Eccles

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