Overexpression of eukaryotic translation initiation factor 5A2 (EIF5A2) correlates with cell aggressiveness and poor survival in gastric cancer

PloS One
Qing-Bin MengWei-Xun Zhou

Abstract

Eukaryotic translation initiation factor 5A2 (EIF5A2) plays an important role in tumor progression and prognosis evaluation. However, little information is available about its potential role in gastric cancer. This study aimed to investigate the function of EIF5A2 in tumor progression and its potential mechanisms. EIF5A2 expression was measured in human gastric cancer cell lines, the immortalized gastric mucosal epithelial cell line (GES-1) and human gastric cancer tissues and knocked down by RNA interference or upregulated by EIF5A2 plasmid transfection. Cell proliferation, migration and invasion were assessed in vitro. The downstream targets of EIF5A2 were examined by western blotting. EIF5A2 and its potential target metastasis-associated protein 1 (MTA1) expression were examined in 160 pairs of human gastric cancer and adjacent non-tumor specimens using immunohistochemistry (IHC) staining, and its correlation with clinicopathological features and survival was investigated. Knockdown of EIF5A2 or MTA1 caused an apparent suppression of HGC27 cell proliferation, migration and invasion. After knockdown of EIF5A2 in HGC27 cells, E-cadherin levels were upregulated and vimentin, cyclin D1, cyclin D3, C-MYC and MTA1 levels were down...Continue Reading

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Citations

Jan 13, 2016·Advances in Biological Regulation·Alan P FieldsNicole R Murray
May 17, 2015·Biochimica Et Biophysica Acta·Michael B Mathews, John W B Hershey
Aug 27, 2015·World Journal of Gastroenterology : WJG·Shu-Bo TianChao Yan
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Jun 27, 2019·World Journal of Gastrointestinal Oncology·Qing-Bin MengWei-Ming Kang
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May 12, 2021·Cellular Signalling·Lilas AlboushiSeyed Mehdi Jafarnejad

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Methods Mentioned

BETA
surgical resection
transfection
PCR
protein assay
electrophoresis

Software Mentioned

SPSS

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