Overexpression of galectin-7 in mouse epidermis leads to loss of cell junctions and defective skin repair

PloS One
Gaëlle GendronneauFrançoise Poirier

Abstract

The proteins of the galectin family are implicated in many cellular processes, including cell interactions, polarity, intracellular trafficking, and signal transduction. In human and mouse, galectin-7 is almost exclusively expressed in stratified epithelia, notably in the epidermis. Galectin-7 expression is also altered in several human tumors of epithelial origin. This study aimed at dissecting the consequences of galectin-7 overexpression on epidermis structure and functions in vivo. We established transgenic mice specifically overexpressing galectin-7 in the basal epidermal keratinocytes and analyzed the consequences on untreated skin and after UVB irradiation or mechanical injury. The intercellular cohesion of the epidermis is impaired in transgenic animals, with gaps developing between adjacent keratinocytes, associated with loss of adherens junctions. The epidermal architecture is aberrant with perturbations in the multilayered cellular organisation of the tissue, and structural defects in the basement membrane. These transgenic animals displayed a reduced re-epithelialisation potential following superficial wound, due to a defective collective migration of keratinocytes. Finally, a single mild dose of UVB induced an abno...Continue Reading

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Citations

Jun 11, 2015·Médecine sciences : M/S·Tamara AdvedissianMireille Viguier
Dec 3, 2016·PloS One·Andrée-Anne GrossetYves St-Pierre
Dec 8, 2017·Scientific Reports·Tamara AdvedissianFrédérique Deshayes
Oct 18, 2019·Current Neuropharmacology·João Ronielly Campêlo AraújoAna Cristina de Oliveira Monteiro-Moreira
Feb 11, 2018·Experimental Dermatology·Nan-Lin Wu, Fu-Tong Liu
Jun 29, 2018·Mediators of Inflammation·Monica Fengsrud BrinchmannMartin Haugmo Iversen
Dec 20, 2017·International Journal of Molecular Sciences·Tamara AdvedissianMireille Viguier
Jun 22, 2021·Histochemistry and Cell Biology·Anna-Kristin LudwigHans-Joachim Gabius
Jul 29, 2021·The Journal of Dermatology·Nicolás A PintoJuan P Cerliani

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Methods Mentioned

BETA
transgenic
transgenics
confocal microscopy
Transmission electron microscopy
immunoprecipitations
electron
environmental stress
co-immmunoprecipitation

Software Mentioned

StatEL

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