Overexpression of hepatitis delta antigen protects insect cells from baculovirus-induced cytolysis

Biochemical and Biophysical Research Communications
S B HwangY S Kim

Abstract

Hepatitis delta virus (HDV) is a human pathogen causing fulminant hepatitis and liver cirrhosis. HDV has a circular single-stranded RNA genome, which encodes only a single protein, hepatitis delta antigen (HDAg), from the antigenomic strand. Although the functional roles of HDAg have been extensively studied, the molecular mechanism of persistent infection and pathogenesis of HDV are not yet understood. Here we report that overexpressed HDAg protects cells from death in baculovirus-infected insect cells. Using both wild-type and recombinant baculovirus-infected insect cells, we have demonstrated that HDAg inhibited wild-type baculovirus-induced cytolysis and thus extended the survival of virus-infected insect cells. By deletion analysis, we show that N-terminal 25 amino acids are essential for this function. From these data, we suggest that HDAg may play a major role in persistent infection of HDV.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1992·Journal of Virology·W S RyuJ Taylor
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F L ChangD S Chen
Oct 9, 1986·Nature·A KosH Schellekens
Aug 1, 1988·Journal of Virology·L SharmeenJ Taylor
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H N WuM M Lai
Oct 1, 1986·The Journal of Infectious Diseases·K F Bergmann, J L Gerin
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M RizzettoJ L Gerin
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W Ludlow
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E Moran
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K Vousden

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Citations

Sep 12, 2000·Journal of Virology·J ChangJ Taylor
Dec 21, 2004·Journal of Biochemistry and Molecular Biology·Soo Ho ChoiSoon Bong Hwang
May 4, 1999·FEBS Letters·S B Hwang, K J Park
Aug 28, 2009·Molecules and Cells·Chul-Yong ParkSoon B Hwang
Jul 2, 2005·World Journal of Gastroenterology : WJG·Soo-Ho ChoiSoon-B Hwang

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