Overexpression of HMGA2 promotes tongue cancer metastasis through EMT pathway

Journal of Translational Medicine
Xiaopeng ZhaoChaobin Pan

Abstract

Metastasis to long distance organs is the main reason leading to morality of tongue squamous cell carcinoma (TSCC); however, the molecular mechanisms are still unknown. High mobility group AT-hook 2 (HMGA2) is highly expressed in multiple metastatic carcinomas, in which it contributes to cancer progression, metastasis and poor prognosis by upregulating Snail expression and inducing epithelial mesenchymal transition (EMT). This study focuses on investigating the role and mechanism of regulation of HMGA2 in the metastasis of TSCC. HMGA2 mRNA and protein expression were examined in TSCC specimens by quantitative real-time polymerase chain reaction, western blotting and immunohistochemistry (IHC). Western blotting, IHC and immunofluorescence were also used to measure the expression and localization of EMT marker E-Cadherin and Vimentin both in TSCC cells and tissues. Knockdown assay was performed in vitro in TSCC cell lines using small interfering RNAs and the functional assay was carried out to determine the role of HMGA2 in TSCC cell migration and invasion. TSCC mRNA and protein expression were significantly up-regulated in tumor tissues when compared to adjacent non-tumor tissues, and the overexpression of HMGA2 was closely corr...Continue Reading

References

Jul 26, 2002·CA: a Cancer Journal for Clinicians·Brad W Neville, Terry A Day
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Jun 13, 2006·Cancer Cell·Monica FedeleAlfredo Fusco
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Nov 30, 2006·Neoplasia : an International Journal for Oncology Research·Xiaofeng ZhouDavid T Wong
Aug 19, 2007·Nature Methods·Margaret S EbertPhillip A Sharp
Nov 16, 2007·Nature Reviews. Cancer·Alfredo Fusco, Monica Fedele
Mar 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Madhu S KumarTyler Jacks
Apr 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kazuo MotoyamaMasaki Mori
Oct 4, 2008·The Journal of Biological Chemistry·Sylvie ThuaultAristidis Moustakas
Jan 6, 2009·Nature·Alberto Mantovani
Feb 19, 2009·Cancer Research·Ivana De MartinoAlfredo Fusco
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri
Jun 3, 2009·The Journal of Clinical Investigation·Hervé AcloqueM Angela Nieto
Nov 26, 2009·Nature Cell Biology·Ulrich WellnerThomas Brabletz
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Feb 10, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Francisco J CivantosJeffrey N Myers
May 25, 2010·Nature Reviews. Cancer·Hiroaki Ikushima, Kohei Miyazono
Sep 8, 2010·Nature Cell Biology·Muh-Hwa YangKou-Juey Wu
Oct 5, 2010·Nature Cell Biology·Alberto Martin, Amparo Cano
Jan 22, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xiaochen WangYun Yen
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Jan 24, 2012·Cell·Andrew D RhimBen Z Stanger
May 2, 2013·The Journal of Investigative Dermatology·Leon RaskinStephen B Gruber
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Miao SunMarsha R Rosner
Jun 1, 2013·Cancer Research·Asahiro MorishitaKiran Chada
Jan 16, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Keqiang ZhangYun Yen
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Jul 17, 2016·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Siegfried LoeschkePatrick H Warnke
Aug 11, 2016·Biochemical and Biophysical Research Communications·Hong ZhangXiao-Peng Zhao
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Huang PingDonghui Wang
Mar 31, 2017·Clinical Oral Investigations·Hai Xia ZhangZhan Gui Tang
Jul 29, 2017·Thoracic Cancer·Xiaotian GaoZeqing Song
Oct 15, 2018·Journal of Cellular Biochemistry·Sanaz NaghizadehBehzad Baradaran
Jun 27, 2019·Journal of Cellular Physiology·Behzad MansooriBehzad Baradaran
Apr 22, 2017·Oncotarget·Mengyuan LiXiaosheng Wang
May 22, 2019·International Journal of Molecular Sciences·Junki SakataHideki Nakayama
Jun 2, 2018·International Journal of Cell Biology·Vincenzo GiancottiClaudio Rizzi
Jul 18, 2018·International Journal of Molecular Medicine·Jianping ZhangXiaotong Wu
Oct 30, 2016·Journal of Experimental & Clinical Cancer Research : CR·Qing-Xuan WangXiao-Hua Zhang
Jun 4, 2020·Cancer Cell International·Kazutaka OuchiHajime Hosoi
Dec 15, 2020·Cancer Biology & Therapy·Jianxin XuJianxin Lyu
Mar 7, 2021·Genes·Behzad MansooriBehzad Baradaran
Sep 4, 2020·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·Tomoaki TakahashiItaru Endo

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
PCR
reverse transcription PCR
electrophoresis
transfection
reverse transcription-PCR

Software Mentioned

SPSS Standard
AxioVision
SPSS

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.