Overexpression of HOXC10 promotes glioblastoma cell progression to a poor prognosis via the PI3K/AKT signalling pathway

Journal of Drug Targeting
Yong GuanJianjun Song

Abstract

The HOX gene is expressed in neoplasias occurred in multiple tissues, such as the colon, lung and breast. However, the effects of the HOX gene on glioblastoma (GBM) remain poorly understood. We examined HOXC10 expression in GBM tissues and cells, analysed its effect on GBM prognosis, and finally assessed its possible underlying mechanisms in this study. HOXC10 expression levels and its prognostic effects on GBM tissues were analysed based on The Cancer Genome Atlas (TCGA) and ONCOMINE database. Overall survival (OS) analysis was performed using the Kaplan-Meier method and log rank test. Then, the expression of HOXC10 was detected in four GBM cell lines using quantitative real-time reverse transcription-PCR (qRT-PCR). In addition, small interfering RNA (si-RNA) was utilised in the U87 cell line with the highest HOXC10 expression to facilitate subsequent in vitro cell experiment. Cell proliferation, migration and invasion were assessed using the Cell Counting Kit-8 (CCK-8) and colony formation assay, wound healing, Transwell assay, respectively in GBM U87 cell after HOXC10 knockdown. Key proteins related to the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) signalling pathway were measured by western blotting. HOXC10...Continue Reading

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Citations

Apr 3, 2020·Cellular and Molecular Life Sciences : CMLS·Céline S GonçalvesBruno M Costa
Apr 7, 2020·Future Oncology·Guohong LiuHaibo Xu
Apr 12, 2019·Frontiers in Molecular Neuroscience·Weiyang LouWeimin Fan
Jun 12, 2021·Frontiers in Oncology·Jinyong FangWenxia Xu
Oct 8, 2021·Cell Biology and Toxicology·U Sangeetha ShenoyRaghu Radhakrishnan

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