Overexpression of HSF2-beta inhibits hemin-induced heat shock gene expression and erythroid differentiation in K562 cells.

The Journal of Biological Chemistry
S LeppäLea Sistonen

Abstract

Acquisition of heat shock factor 2 (HSF2) DNA binding activity is accompanied by induced transcription of heat shock genes in hemin-treated K562 cells undergoing erythroid differentiation. Previous studies revealed that HSF2 consists of two alternatively spliced isoforms, HSF2-alpha and HSF2-beta, whose relative abundance is developmentally regulated and varies between different tissues. To investigate whether the molar ratio of HSF2-alpha and HSF2-beta isoforms is crucial for the activation of HSF2 and whether the HSF2 isoforms play functionally distinct roles during the hemin-mediated erythroid differentiation, we generated cell clones expressing different levels of HSF2-alpha and HSF2-beta. We show that in parental K562 cells, the HSF2-alpha isoform is predominantly expressed and HSF2 can be activated upon hemin treatment. In contrast, when HSF2-beta is expressed at levels exceeding those of endogenous HSF2-alpha, the hemin-induced DNA binding activity and transcription of heat shock genes are repressed, whereas overexpression of HSF2-alpha results in an enhanced hemin response. Furthermore, the hemin-induced accumulation of globin, known as a marker of erythroid differentiation, is decreased in cells overexpressing HSF2-bet...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K RabindranC Wu
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T J SchuetzR E Kingston
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J YenI M Verma
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P GunningL Kedes
Jun 1, 1989·Molecular and Cellular Biology·E HickeyL A Weber
Dec 1, 1989·Trends in Biochemical Sciences·G PadmanabanP N Rangarajan
Feb 1, 1985·Molecular and Cellular Biology·B WuR Morimoto
Sep 1, 1988·Molecular and Cellular Biology·J AminR Voellmy
Nov 1, 1984·Molecular and Cellular Biology·S S BanerjiR I Morimoto
Apr 21, 1995·The Journal of Biological Chemistry·M T HaldemanC M Pickart
Dec 1, 1994·Developmental Biology·V MezgerJ P Renard
Feb 11, 1995·Nucleic Acids Research·M T FiorenzaV Zimarino
Aug 1, 1994·Molecular and Cellular Biology·S P MurphyB Phillips
Jan 1, 1995·Annual Review of Cell and Developmental Biology·C Wu

❮ Previous
Next ❯

Citations

Mar 10, 2000·Biochemical and Biophysical Research Communications·L PaslaruM Morange
Mar 17, 2004·Experimental Cell Research·Pascale Le GoffDenis Michel
May 15, 2003·Biochemical and Biophysical Research Communications·Keisuke OkitaTetsuya Taga
Oct 10, 2002·Hearing Research·Damon A FairfieldRichard A Altschuler
Feb 20, 2003·Neurobiology of Learning and Memory·José M PizarroEdwin J Barea-Rodriguez
Oct 8, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Susanna AiraksinenMikko Nikinmaa
Mar 25, 2000·Molecular and Cellular Biology·L PirkkalaL Sistonen
Jan 25, 2002·Cell Stress & Chaperones·P NykänenL Sistonen
Feb 28, 2006·Inflammation·Sabrina M Heidemann, Maria Glibetic
Feb 7, 2015·Cell Stress & Chaperones·Shardul Vikram LalSachinandan De
Dec 31, 1997·The Journal of Biological Chemistry·S LeppäL Sistonen
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L PirkkalaL Sistonen
Nov 26, 1998·The Journal of Biological Chemistry·Y ZhangN F Mivechi
Sep 17, 1998·The Journal of Biological Chemistry·T YoshimaH Yanagi
Jun 19, 2003·The Journal of Biological Chemistry·Haiying HeStuart K Calderwood
May 25, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L PirkkalaL Sistonen
Oct 8, 2021·Journal of Cellular Physiology·Vasiliki-Dimitra C TsolakiAsterios S Tsiftsoglou

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.