Overexpression of Human CD55 and CD59 or Treatment with Human CD55 Protects against Renal Ischemia-Reperfusion Injury in Mice

The Journal of Immunology : Official Journal of the American Association of Immunologists
Anjan K BongoniP J Cowan

Abstract

Deficiency in the membrane-bound complement regulators CD55 and CD59 exacerbates renal ischemia-reperfusion injury (IRI) in mouse models, but the effect of increasing CD55 and CD59 activity has not been examined. In this study, we investigated the impact of overexpression of human (h) CD55 ± hCD59 or treatment with soluble rhCD55 in a mouse model of renal IRI. Unilaterally nephrectomised mice were subjected to 18 (mild IRI) or 22 min (moderate IRI) warm renal ischemia, and analyzed 24 h after reperfusion for renal function (serum creatinine and urea), complement deposition (C3b/c and C9), and infiltration of neutrophils and macrophages. Transgenic mice expressing hCD55 alone were protected against mild renal IRI, with reduced creatinine and urea levels compared with wild type littermates. However, the renal function of the hCD55 mice was not preserved in the moderate IRI model, despite a reduction in C3b/c and C9 deposition and innate cell infiltration. Mice expressing both hCD55 and hCD59, on the other hand, were protected in the moderate IRI model, with significant reductions in all parameters measured. Wild type mice treated with rhCD55 immediately after reperfusion were also protected in the moderate IRI model. Thus, manipu...Continue Reading

References

Aug 4, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·M HeinzelmannJ C Passmore
May 17, 2000·The Journal of Clinical Investigation·W ZhouS H Sacks
Jun 4, 2002·Nature Medicine·Julian R PrattSteven H Sacks
Dec 11, 2002·Kidney International·Thiruma V ArumugamStephen M Taylor
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bart de VriesWim A Buurman
Oct 8, 2003·The Journal of Experimental Medicine·Donald W LawrenceSean P Colgan
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Koei YamadaWen-Chao Song
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sivasankar BaalasubramanianB Paul Morgan
Nov 25, 2004·American Journal of Physiology. Renal Physiology·Yuan-Ji DayMark D Okusa
Jun 10, 2005·Xenotransplantation·B Paul MorganClaire L Harris
Jan 31, 2006·The Journal of Clinical Investigation·Joshua M ThurmanV Michael Holers
Jun 27, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·X ZhengW-P Min
Nov 30, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Zeynep AydinTon J Rabelink
Jun 29, 2007·American Journal of Physiology. Renal Physiology·H Thomas LeeCharles W Emala
Jul 17, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Christine WeeksGeorge C Tsokos
Sep 15, 2007·The Journal of Clinical Investigation·Huiling WuSteven J Chadban
Apr 15, 2008·Transplantation·Jeffrey DammanMarc A Seelen
Sep 6, 2008·The American Journal of Pathology·Xiufen ZhengWei-Ping Min
Dec 24, 2008·Transplantation·Bo LuKaren M Dwyer
Feb 26, 2010·Mediators of Inflammation·Ali AkcayCharles L Edelstein
Aug 20, 2010·Nature Immunology·Daniel RicklinJohn D Lambris
Sep 28, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Umme AmaraMarkus Huber-Lang
Oct 6, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Joshua M Thurman, Brandon Renner
Jul 17, 2012·Journal of the American Society of Nephrology : JASN·Qi PengWuding Zhou
Apr 30, 2016·Circulation Research·Jonathan H Foley, Edward M Conway

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Citations

Nov 5, 2017·Biochemical Society Transactions·Bernd Gesslbauer, Valery Bochkov
Nov 16, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chao HuWeiguo Hu
Apr 3, 2020·Journal of Translational Medicine·Mohammad Afzal KhanDieter Clemens Broering
Oct 30, 2018·Journal of the American Heart Association·Yuntian ShenWei Yang
Sep 12, 2019·Clinical and Experimental Immunology·J VolkmannS von Vietinghoff
May 28, 2020·Frontiers in Physiology·Laura KummerJan Larmann

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