PMID: 15350186Sep 8, 2004Paper

Overexpression of human IGF-II mRNA in the brain of transgenic mice modulates IGFBP-2 gene expression in the medulla oblongata

The Journal of Endocrinology
C M A ReijndersS C van Buul-Offers

Abstract

The insulin-like growth factors, IGF-I and IGF-II, and their binding proteins play an important role in the growth and development of the central nervous system. In the brain, colocalization of IGFs and IGFBPs often occurs, suggesting that IGFBPs can modulate IGF action. In one strain of our human (h)IGF-II transgenic mice, which carry an hIGF-II transgene driven by the H-2Kb promoter, we found overexpression of hIGF-II in the brain, as measured by Northern blot analysis. To clarify the localization and influence of the hIGF-II transgene on different components of the GH-IGF axis in the brain, we studied the expression pattern of the hIGF-II transgene, endogenous IGF-I and IGF-II, and IGFBP-2, -3 and -5 in the brain of prepubertal 4-week-old mice, using nonradioactive in situ hybridization. We found that the hIGF-II transgene is exclusively expressed in neurons of the piriform cortex, the cerebral cortex, the medulla oblongata and the granular layer of the cerebellum. In general, this pattern is comparable to the expression pattern of endogenous IGF-I, with a few exceptions: there is no expression of IGF-I in the granular layer of the cerebellum, whereas the Purkinje cells of the cerebellum and thalamus both express IGF-I but n...Continue Reading

Citations

Mar 4, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Ruo L ChenJane E Preston
Feb 3, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Sylvia C van Buul-OffersMarcel Karperien
Jan 24, 2007·The European Journal of Neuroscience·Jeannette HenningUlrike Gimsa
Jun 20, 2012·Frontiers in Neuroendocrinology·John O'Kusky, Ping Ye
Apr 30, 2014·Frontiers in Endocrinology·Andreas HoeflichJulia Brenmoehl
Dec 12, 2019·Frontiers in Endocrinology·Shumsuzzaman Khan

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