PMID: 7537791Mar 1, 1995Paper

Overexpression of human insulin-like growth factor-II in transgenic mice causes increased growth of the thymus

The Journal of Endocrinology
S C van Buul-OffersJ L Van den Brande

Abstract

In order to determine the effects of IGF-II overexpression on growth of mice, transgenic mice were produced carrying one of three different H-2Kb human IGF-II minigenes in which different non-coding exons (exon 5, truncated exon 5 or exon 6) preceded the coding exons 7, 8 and 9. These were spaced by truncated introns and for proper polyadenylation an SV40 polyadenylation signal was incorporated. The highest levels of IGF-II minigene mRNA expression were found in lines containing the truncated exon 5 construct (II5'). Those containing exon 6 (II6) had less expression and 5 constructs (II5) gave only moderate levels of mRNA expression. In general mRNA expression was highest in thymus and spleen, low in liver and kidney and absent in the brain. In addition, one II5' line showed expression in the brain. Serum IGF-II levels at 8 weeks of age were increased 7- to 8-fold in homozygous transgenic lines with construct II5' without brain expression and 2- to 3-fold in the one that showed expression in the brain; serum IGF-I levels were unchanged. Serum IGFs in the lines containing the constructs II5 and II6 were not different from those of the controls. In all cases body length and weight as well as the weight of several organs such as b...Continue Reading

Citations

May 8, 1998·The Journal of Experimental Zoology·D D Pravtcheva, T L Wise
Sep 18, 1997·Molecular Reproduction and Development·T L Wise, D D Pravatcheva
Oct 20, 2010·Nature Reviews. Endocrinology·Peter E ClaytonAndrew G Renehan
May 3, 2000·Pathology International·C YeM Matsuyama
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Abbe N VallejoCheryl A Conover
Mar 24, 2005·Neuroimmunomodulation·Wilson SavinoMireille Dardenne
Nov 25, 2003·Microscopy Research and Technique·Olivia García-SuárezGiovanni Germanà
Mar 23, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Louise HarveyDarryl W Eyles
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·E WolfH Lahm
Jun 20, 2012·Frontiers in Neuroendocrinology·John O'Kusky, Ping Ye
Oct 3, 2002·Neuropeptides·A Joseph D'ErcoleJohn R O'Kusky
Aug 19, 2000·Endocrine Reviews·W Savino, M Dardenne
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabelle HansenneVincent Geenen
May 21, 1999·Journal of Neuroendocrinology·O KechaR Winkler
Mar 5, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marco BreinigRalf Joachim Rieker
Sep 1, 1996·Endocrinology and Metabolism Clinics of North America·A J D'Ercole

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