Overexpression of Human Papillomavirus Type 16 Oncoproteins Enhances Epithelial-Mesenchymal Transition via STAT3 Signaling Pathway in Non-Small Cell Lung Cancer Cells.

Oncology Research
Wenzhang ZhangXudong Tang

Abstract

The human papillomavirus (HPV) infection may be associated with the development and progression of non-small cell lung cancer (NSCLC). However, the role of HPV-16 oncoproteins in the development and progression of NSCLC is not completely clear. Epithelial-mesenchymal transition (EMT), a crucial step for invasion and metastasis, plays a key role in the development and progression of NSCLC. Here we explored the effect of HPV-16 oncoproteins on EMT and the underlying mechanisms. NSCLC cell lines, A549 and NCI-H460, were transiently transfected with the EGFP-N1-HPV-16 E6 or E7 plasmid. Real-time PCR and Western blot analysis were performed to analyze the expression of EMT markers. A protein microarray was used to screen the involved signaling pathway. Our results showed that overexpression of HPV-16 E6 and E7 oncoproteins in NSCLC cells significantly promoted EMT-like morphologic changes, downregulated the mRNA and protein levels of EMT epithelial markers (E-cadherin and ZO-1), and upregulated the mRNA and protein levels of EMT mesenchymal markers (N-cadherin and vimentin) and transcription factors (ZEB-1 and Snail-1). Furthermore, the HPV-16 E6 oncoprotein promoted STAT3 activation. Moreover, WP1066, a specific signal transducer a...Continue Reading

Citations

Jan 27, 2018·Journal of Cancer Research and Clinical Oncology·Ming-Ming JiangQing-Guo Di
May 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sen LinHongbo Wang
Mar 22, 2021·Journal of Cutaneous Pathology·Robert F MooreCharles G Eberhart
Jun 29, 2021·Genes & Diseases·Yong HuangHongtao Ren
May 1, 2018·Current Problems in Cancer·Shilpi GuptaBhudev C Das
Jun 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuan YuanHe Xin

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Methods Mentioned

BETA
PCR
transfection
flow cytometry
X-ray

Software Mentioned

SPSS

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