Abstract
The human papillomavirus (HPV) infection may be associated with the development and progression of non-small cell lung cancer (NSCLC). However, the role of HPV-16 oncoproteins in the development and progression of NSCLC is not completely clear. Epithelial-mesenchymal transition (EMT), a crucial step for invasion and metastasis, plays a key role in the development and progression of NSCLC. Here we explored the effect of HPV-16 oncoproteins on EMT and the underlying mechanisms. NSCLC cell lines, A549 and NCI-H460, were transiently transfected with the EGFP-N1-HPV-16 E6 or E7 plasmid. Real-time PCR and Western blot analysis were performed to analyze the expression of EMT markers. A protein microarray was used to screen the involved signaling pathway. Our results showed that overexpression of HPV-16 E6 and E7 oncoproteins in NSCLC cells significantly promoted EMT-like morphologic changes, downregulated the mRNA and protein levels of EMT epithelial markers (E-cadherin and ZO-1), and upregulated the mRNA and protein levels of EMT mesenchymal markers (N-cadherin and vimentin) and transcription factors (ZEB-1 and Snail-1). Furthermore, the HPV-16 E6 oncoprotein promoted STAT3 activation. Moreover, WP1066, a specific signal transducer a...Continue Reading
Citations
Apr 10, 2018·Journal of Cellular Physiology·Bing JinBing Li
Jan 27, 2018·Journal of Cancer Research and Clinical Oncology·Ming-Ming JiangQing-Guo Di
Dec 13, 2019·Journal of Clinical Laboratory Analysis·Hui ChenXin Li
Oct 17, 2019·Journal of Clinical Laboratory Analysis·Xiaohong HaoLin Cong
Jun 8, 2019·Journal of Cellular Biochemistry·Yuanbo ZhongAijun Shan
Mar 7, 2020·Journal of Clinical Laboratory Analysis·Tao Fang, Qi Lu
Nov 30, 2019·Journal of Clinical Laboratory Analysis·Yingchao LiuYaozeng Xie
May 2, 2020·Journal of Clinical Laboratory Analysis·Yan YanJie Gao
May 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sen LinHongbo Wang
Jul 10, 2020·Journal of Clinical Laboratory Analysis·Luqing ZhangCui Ma
Jul 2, 2019·Molecular Medicine Reports·Yao ZhangXia Li
Jul 17, 2020·Journal of Clinical Laboratory Analysis·Rongbo LinYunbin Ye
Jul 20, 2018·Respiratory Research·Nathan Rout-PittMartin Donnelley
Oct 21, 2020·Cells·Adriana Gutiérrez-Hoya, Isabel Soto-Cruz
Jan 26, 2021·Frontiers in Immunology·Zhen DongHongjuan Cui
Mar 22, 2021·Journal of Cutaneous Pathology·Robert F MooreCharles G Eberhart
Jun 29, 2021·Genes & Diseases·Yong HuangHongtao Ren
May 1, 2018·Current Problems in Cancer·Shilpi GuptaBhudev C Das
Jun 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuan YuanHe Xin