Overexpression of HvIcy6 in Barley Enhances Resistance against Tetranychus urticae and Entails Partial Transcriptomic Reprogramming

International Journal of Molecular Sciences
M Estrella SantamariaManuel Martínez

Abstract

Cystatins have been largely used for pest control against phytophagous species. However, cystatins have not been commonly overexpressed in its cognate plant species to test their pesticide capacity. Since the inhibitory role of barley HvCPI-6 cystatin against the phytophagous mite Tetranychus urticae has been previously demonstrated, the purpose of our study was to determine if barley transgenic lines overexpressing its own HvIcy6 gene were more resistant against this phytophagous infestation. Besides, a transcriptomic analysis was done to find differential expressed genes among wild-type and transformed barley plants. Barley plants overexpressing HvIcy6 cystatin gene remained less susceptible to T. urticae attack when compared to wild-type plants, with a significant lesser foliar damaged area and a lower presence of the mite. Transcriptomic analysis revealed a certain reprogramming of cellular metabolism and a lower expression of several genes related to photosynthetic activity. Therefore, although caution should be taken to discard potential deleterious pleiotropic effects, cystatins may be used as transgenes with impact on agricultural crops by conferring enhanced levels of resistance to phytophagous pests.

References

Feb 8, 2002·Trends in Plant Science·Martin Heil, Ian T Baldwin
Jan 7, 2004·The Biochemical Journal·Neil D RawlingsAlan J Barrett
Oct 22, 2008·BMC Research Notes·Luis Oñate-Sánchez, Jesús Vicente-Carbajosa
Jul 9, 2009·International Journal of Plant Genomics·Goetz HenselJochen Kumlehn
May 7, 2010·Plant, Cell & Environment·Damla D BilginEvan H DeLucia
Jun 19, 2010·Biochimie·Meriem BenchabaneDominique Michaud
Jun 29, 2010·Journal of Experimental Botany·Urte SchlüterDominique Michaud
Mar 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anne KempelMark van Kleunen
Nov 25, 2011·Nature·Miodrag GrbićYves Van de Peer
Jan 10, 2012·Physiologia Plantarum·Manuel MartínezIsabel Díaz
Dec 4, 2012·Nucleic Acids Research·Thomas NussbaumerManuel Spannagl
May 23, 2013·Scientific Reports·Mohamed AliGen-ichiro Arimura
Jan 30, 2014·Annual Review of Plant Biology·Carlos L Ballaré
Apr 30, 2014·Molecular Plant·Bethany HuotSheng Yang He
Oct 11, 2014·Methods in Molecular Biology·Cornelia MartheGoetz Hensel
Oct 24, 2014·Annual Review of Entomology·Keyan Zhu-Salzman, Rensen Zeng
May 12, 2015·Journal of Insect Physiology·María E SantamaríaFélix Ortego
Mar 20, 2016·Plant Science : an International Journal of Experimental Plant Biology·Stefan G van WykBarend J Vorster
Apr 5, 2016·Nature Biotechnology·Nicolas L BrayLior Pachter
May 25, 2016·Journal of Experimental Botany·Blanca Velasco-ArroyoIsabel Diaz
Oct 25, 2016·International Journal of Molecular Sciences·Manuel MartinezIsabel Diaz
Feb 2, 2017·Annual Review of Plant Biology·Tobias Züst, Anurag A Agrawal
Mar 23, 2017·The Plant Cell·Talia L KarasovJoy Bergelson
Jun 6, 2017·Nature Methods·Harold PimentelLior Pachter
Sep 29, 2017·Frontiers in Plant Science·Mercedes Diaz-MendozaManuel Martinez

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Citations

Jul 26, 2018·Frontiers in Plant Science·Ana ArnaizM E Santamaria
Aug 22, 2018·International Journal of Molecular Sciences·Isabel Diaz

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Methods Mentioned

BETA
transgenic
RNA-seq
PCR
reverse-transcription quantitative PCR
Illumina sequencing

Software Mentioned

Adobe Photoshop
Heatmapper
Kallisto
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