Overexpression of Insulin-Like Growth Factor 1 Enhanced the Osteogenic Capability of Aging Bone Marrow Mesenchymal Stem Cells

Theranostics
Ching-Yun ChenShankung Lin

Abstract

Many studies have indicated that loss of the osteoblastogenic potential in bone marrow mesenchymal stem cells (bmMSCs) is the major component in the etiology of the aging-related bone deficit. But how the bmMSCs lose osteogenic capability in aging is unclear. Using 2-dimentional cultures, we examined the dose response of human bmMSCs, isolated from adult and aged donors, to exogenous insulin-like growth factor 1 (IGF-1), a growth factor regulating bone formation. The data showed that the mitogenic activity and the osteoblastogenic potential of bmMSCs in response to IGF-1 were impaired with aging, whereas higher doses of IGF-1 increased the proliferation rate and osteogenic potential of aging bmMSCs. Subsequently, we seeded IGF-1-overexpressing aging bmMSCs into calcium-alginate scaffolds and incubated in a bioreactor with constant perfusion for varying time periods to examine the effect of IGF-1 overexpression to the bone-forming capability of aging bmMSCs. We found that IGF-1 overexpression in aging bmMSCs facilitated the formation of cell clusters in scaffolds, increased the cell survival inside the cell clusters, induced the expression of osteoblast markers, and enhanced the biomineralization of cell clusters. These results ...Continue Reading

Citations

Oct 6, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Faming TianDaniel D Bikle
Mar 28, 2018·Journal of Molecular Endocrinology·Richard C LindseySubburaman Mohan
Mar 15, 2019·Journal of Oral Rehabilitation·Yanjing LiYunfeng Lin
Jul 8, 2020·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Rui JiangTong Zhang
May 28, 2020·International Journal of Molecular Sciences·Addolorata CorradoFrancesco Paolo Cantatore
Jan 18, 2020·Stem Cells International·Sushmitha S DurgamLaura D Hostnik
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Xueke ZhouXin Li
Dec 20, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Liping ZhouMan-Sau Wong
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Pei-Chin ChenYen-Hua Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
chemical treatments
PCR
transfection
Assay
reverse transcription-PCRs
infrared spectroscopy
X-ray
glycosylation

Software Mentioned

Imaris Scientific 3D Image Processing & Analysis
Imaris Scientific 3D
MetaMorph Microscopy Automation Image Analysis
Crystallography Open Database ( COD )
ZEN lite
Integrated Spectral Data Base System for Organic Compounds ( S...

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.