Overexpression of integrin-linked kinase (ILK) promotes migration and invasion of colorectal cancer cells by inducing epithelial-mesenchymal transition via NF-κB signaling

Acta histochemica
Zhaopeng YanQi Su

Abstract

Integrin-linked kinase (ILK), a ubiquitously expressed and evolutionally conserved serine/threonine kinase, has been shown to be aberrantly overexpressed and activated in diversified types of human malignancies, including colorectal cancer (CRC). However, the potential role of ILK in cancer cell migration and invasion remains to be elucidated. In this study, we introduced the human ILK gene into a low ILK-expressing human CRC cell line SW480. Cell migration and invasion were evaluated by the wound healing assay and transwell invasion assay, respectively. The epithelial-mesenchymal transition (EMT)-related proteins were detected by Western blot analysis or immunofluorescence. We found that enforced overexpression of ILK in SW480 cells dramatically promoted their migratory and invasive ability in vitro. Furthermore, SW480 cells stably overexpressing ILK underwent EMT, as indicated by mesenchymal morphology, decreased expression of E-cadherin, and increased expression of vimentin, Snail, and Slug. Finally, the nuclear factor (NF)-κB inhibitor BAY 11-7028 or NF-κB p65 small interfering RNA significantly restored the reduced E-cadherin level in ILK-overexpressing cells, suggesting that ILK-mediated down-regulation of E-cadherin is d...Continue Reading

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Citations

Aug 5, 2015·Cell Adhesion & Migration·Wenting XuNonghua Lu
May 13, 2015·Journal of the Egyptian National Cancer Institute·Dalia M Abd El-RehimMaiiada H Nazmy
Sep 6, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Qiao-Mei ZhengLi-Hong Chen
Jun 28, 2017·Proceedings of the National Academy of Sciences of the United States of America·Moustafa R K AliMostafa A El-Sayed
Mar 10, 2019·Nature Reviews. Drug Discovery·Jennifer E Kung, Natalia Jura
Jul 9, 2021·Journal of Experimental & Clinical Cancer Research : CR·Snehangshu KunduTobias Sjöblom

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